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Plant Physiol, December 2001, Vol. 127, pp. 1739-1749

DEX1, a Novel Plant Protein, Is Required for Exine Pattern Formation during Pollen Development in Arabidopsis1

Dawn M. Paxson-Sowders,2 Craig H. Dodrill, Heather A. Owen,3 and Christopher A. Makaroff*

Department of Chemistry and Biochemistry, Miami University, Oxford, Ohio 45056

To identify factors that are required for proper pollen wall formation, we have characterized the T-DNA-tagged, dex1 mutation of Arabidopsis, which results in defective pollen wall pattern formation. This study reports the isolation and molecular characterization of DEX1 and morphological and ultrastructural analyses of dex1 plants. DEX1 encodes a novel plant protein that is predicted to be membrane associated and contains several potential calcium-binding domains. Pollen wall development in dex1 plants parallels that of wild-type plants until the early tetrad stage. In dex1 plants, primexine deposition is delayed and significantly reduced. The normal rippling of the plasma membrane and production of spacers observed in wild-type plants is also absent in the mutant. Sporopollenin is produced and randomly deposited on the plasma membrane in dex1 plants. However, it does not appear to be anchored to the microspore and forms large aggregates on the developing microspore and the locule walls. Based on the structure of DEX1 and the phenotype of dex1 plants, several potential roles for the protein are proposed.

1 This work was supported by the U.S. Department of Agriculture (grant no. 95-37304-2246) and by the National Research Initiative Competitive Grants Program (to C.A.M.).

2 Present address: Department of Rheumatology, Children's Hospital Medical Center, Cincinnati, OH 45229.

3 Present address: Department of Biological Sciences, University of Wisconsin, Milwaukee, WI 53211.

* Corresponding author; e-mail: makaroca{at}muohio.edu; fax 513-529-5715.

© 2001 American Society of Plant Physiologists


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