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Plant Physiol, December 2001, Vol. 127, pp. 1750-1763
Different Myrosinase and Idioblast Distribution in Arabidopsis
and Brassica napus1
Erik
Andréasson,*
Lise Bolt
Jørgensen,
Anna-Stina
Höglund,
Lars
Rask, and
Johan
Meijer
Department of Plant Physiology, Institute of Molecular Biology,
University of Copenhagen, Oester Farimagsgade 2A, DK-1353 Copenhagen,
Denmark (E.A.); Department of Evolutionary Botany, Botanical Institute,
University of Copenhagen, Gothersgade 140, DK-1123 Copenhagen, Denmark
(L.B.J.); Department of Plant Biology, Uppsala Genetic Center, Swedish
University of Agricultural Sciences, Box 7080, S-750 07 Uppsala,
Sweden (A.-S.H., J.M.); and Department of Medical Biochemistry and
Microbiology, Biomedical Centre, Box 582, Uppsala University, S-751 23 Uppsala, Sweden (L.R.)
Myrosinase (EC 3.2.3.1) is a glucosinolate-degrading enzyme mainly
found in special idioblasts, myrosin cells, in Brassicaceae. This
two-component system of secondary products and degradative enzymes is
important in plant-insect interactions. Immunocytochemical analysis of
Arabidopsis localized myrosinase exclusively to myrosin cells in the
phloem parenchyma, whereas no myrosin cells were detected in the ground
tissue. In Brassica napus, myrosinase could be detected
in myrosin cells both in the phloem parenchyma and in the ground
tissue. The myrosin cells were similar in Arabidopsis and B.
napus and were found to be different from the companion cells
and the glucosinolate-containing S-cells present in Arabidopsis. Confocal laser scanning immunomicroscopy analysis of myrosin cells in
B. napus embryos showed that the myrosin grains
constitute a continuous reticular system in the cell. These findings
indicate that in the two species studied, initial cells creating the
ground tissue have different potential for making idioblasts and
suggest that the myrosinase-glucosinolate system has at least partly
different functions. Several myrosinases in B. napus
extracts are recovered in complex together with myrosinase-binding
protein (MBP), and the localization of MBP was therefore studied in
situ. The expression of MBP was highest in germinating seedlings of
B. napus and was found in every cell except the myrosin
cells of the ground tissue. Rapid disappearance of the MBP from the
non-myrosin cells and emergence of MBP in the myrosin cells resulted in
an apparent colocalization of MBP and myrosinase in 7-d-old seedlings.
1
This study was supported by grants from the
Nordic Joint Committee for Agricultural Research, from the Swedish
Council for Forestry and Agricultural Research, from the Swedish
Foundation for Strategic Research, from Nilsson-Ehles, and from Lamms Stiftelse.
*
Corresponding author; e-mail Erik.Andreasson{at}my.molbio.ku.dk;
fax 45-353-221-28.
© 2001 American Society of Plant Physiologists
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