|
Plant Physiol, December 2001, Vol. 127, pp. 1808-1818
Conditional Circadian Regulation of PHYTOCHROME A
Gene Expression
Anthony
Hall,
László
Kozma-Bognár,
Réka
Tóth,
Ferenc
Nagy, and
Andrew J.
Millar*
Department of Biological Sciences, University of Warwick, Coventry
CV4 7AL, United Kingdom (A.H., A.J.M.); and Plant Biology Institute,
Biological Research Center of the Hungarian Academy of Sciences,
P.O. Box 521, H-6701 Szeged, Hungary (L.K.-B., R.T., F.N.)
The phytochrome photoreceptors and the circadian clock control many
of the same developmental processes, in all organs and throughout the
growth of Arabidopsis plants. Phytochrome A (phyA) provides light input
signals to entrain the circadian clock. The clock is known to
rhythmically regulate its light input pathway, so we tested rhythmic
regulation of phyA, using transgenic plants carrying a
PHYA promoter fusion to the luciferase reporter
(PHYA:LUC). We provide the first images of
LUC activity with subcellular resolution in intact
tissue. PHYA transcription and the accumulation of all three PHYA mRNAs were indeed clock controlled.
PHYA is expressed throughout the seedling, so we tested
whether circadian rhythms were observed in all
PHYA-expressing organs and whether the rhythms were
autonomously controlled by each organ. In contrast to our previous
results using other clock controlled genes, the rhythmic pattern of
PHYA expression varied markedly among isolated organs and between isolated organs and intact plants. High-amplitude rhythms
were maintained for many days in isolated leaves in darkness, whereas
the leaves of intact plants rapidly lost rhythmicity. Wounding the
leaves of intact plants had no effect. The rhythmic pattern of
PHYA expression is not organ autonomous but depends upon
the physical continuity or isolation of the rhythmic tissues, consistent with the presence of a transmitted signal that controls the
overt expression of circadian rhythms without necessarily affecting the
underlying clock. A circadian system might be present in most, if not
all, plant cells, but its effect on intracellular rhythms can be
controlled by supracellular signaling.
*
Corresponding author; e-mail andrew.millar{at}warwick.ac.uk; fax
44-024-7652 3701.
© 2001 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
Z. Ding, M. R. Doyle, R. M. Amasino, and S. J. Davis
A Complex Genetic Interaction Between Arabidopsis thaliana TOC1 and CCA1/LHY in Driving the Circadian Clock and in Output Regulation
Genetics,
July 1, 2007;
176(3):
1501 - 1510.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Imaizumi, J. I. Schroeder, and S. A. Kay
In SYNC: The Ins and Outs of Circadian Oscillations in Calcium
Sci. Signal.,
June 12, 2007;
2007(390):
pe32 - pe32.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Kevei, P. Gyula, A. Hall, L. Kozma-Bognar, W.-Y. Kim, M. E. Eriksson, R. Toth, S. Hanano, B. Feher, M. M. Southern, et al.
Forward Genetic Analysis of the Circadian Clock Separates the Multiple Functions of ZEITLUPE
Plant Physiology,
March 1, 2006;
140(3):
933 - 945.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Viczian, S. Kircher, E. Fejes, A. J. Millar, E. Schafer, L. Kozma-Bognar, and F. Nagy
Functional Characterization of Phytochrome Interacting Factor 3 for the Arabidopsis thaliana Circadian Clockwork
Plant Cell Physiol.,
October 1, 2005;
46(10):
1591 - 1602.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Sullivan, G. I. Jenkins, and H. G. Nimmo
Roots, Cycles and Leaves. Expression of the Phosphoenolpyruvate Carboxylase Kinase Gene Family in Soybean
Plant Physiology,
August 1, 2004;
135(4):
2078 - 2087.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. Millar
Input signals to the plant circadian clock
J. Exp. Bot.,
January 2, 2004;
55(395):
277 - 283.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. Millar
A Suite of Photoreceptors Entrains the Plant Circadian Clock
J Biol Rhythms,
June 1, 2003;
18(3):
217 - 226.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J. Z. Kiss, J. L. Mullen, M. J. Correll, and R. P. Hangarter
Phytochromes A and B Mediate Red-Light-Induced Positive Phototropism in Roots
Plant Physiology,
March 1, 2003;
131(3):
1411 - 1417.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. P. Michael and C. R. McClung
Phase-Specific Circadian Clock Regulatory Elements in Arabidopsis
Plant Physiology,
October 1, 2002;
130(2):
627 - 638.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. C. Thain, G. Murtas, J. R. Lynn, Robert. B. McGrath, and A. J. Millar
The Circadian Clock That Controls Gene Expression in Arabidopsis Is Tissue Specific
Plant Physiology,
September 1, 2002;
130(1):
102 - 110.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Izawa, T. Oikawa, N. Sugiyama, T. Tanisaka, M. Yano, and K. Shimamoto
Phytochrome mediates the external light signal to repress FT orthologs in photoperiodic flowering of rice
Genes & Dev.,
August 1, 2002;
16(15):
2006 - 2020.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
