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Plant Physiol, January 2002, Vol. 128, pp. 201-211
Differential Effect of Jasmonic Acid and Abscisic Acid on Cell
Cycle Progression in Tobacco BY-2 Cells1
Agnieszka
 wi tek,
Marc
Lenjou,
Dirk
Van Bockstaele,
Dirk
Inzé, and
Harry
Van Onckelen*
Laboratory of Plant Physiology and Biochemistry, Department of
Biology, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp,
Belgium (A.S., H.V.O.); Laboratory of Experimental Hematology,
University of Antwerp, Antwerp University Hospital, Wilrijkstraat 10, B-2650 Edegem, Belgium (M.L., D.V.B.); Vakgroep Moleculaire Genetica & Departement Plantengenetica, Vlaams Interuniversitair Instituut voor
Biotechnologie, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000
Gent, Belgium (D.I.)
Environmental stress affects plant growth and development. Several
plant hormones, such as salicylic acid, abscisic acid (ABA), jasmonic
acid (JA), and ethylene play a crucial role in altering plant
morphology in response to stress. Developmental regulation often has
the cell cycle machinery among its targets. We analyzed the effect of
JA and ABA on cell cycle progression in synchronized tobacco
(Nicotiana tabacum) BY-2 cells. Both compounds
were found to prevent DNA replication, keeping the cells in the G1
stage, when applied just before the G1/S transition. However, ABA did not have any effect on subsequent phases of the cell cycle when applied
at a later stage, whereas JA effectively prevented mitosis on
application during DNA synthesis. This demonstrates that JA treatment
can freeze synchronized BY-2 cells in both the G1 and G2 stages of the
cell cycle. Jasmonate administered after the S-phase was less effective
in decreasing the mitotic index, suggesting that cell sensitivity
toward JA is dependent on the cell cycle phase. In cultures detained in
the G2-phase, we observed a reduced histone H1 kinase activity of
kinases associated with the p13sucl protein.
1
This work was supported by Geconcenteerde
Onderzoeks Actie and by the Belgian Program on
Interuniversity Poles of Attraction (Prime Minister's Office, Science
Programming, grant no. 15).
*
Corresponding author; email hvo{at}uia.ua.ac.be; fax
32-3820-2271.
© 2002 American Society of Plant Physiologists
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