|
Plant Physiol, January 2002, Vol. 128, pp. 21-29
An Oligopeptide Transporter Gene Family in
Arabidopsis1
Serry
Koh,2
Amy M.
Wiles,
Joshua S.
Sharp,
Fred R.
Naider,
Jeffrey M.
Becker, and
Gary
Stacey*
Center for Legume Research (S.K., G.S.), Department of Microbiology
(S.K., J.M.B., G.S.), Department of Biochemistry, Cellular, and
Molecular Biology (A.M.W., J.M.B.), and Graduate School of Genome
Science and Technology (J.S.S., J.M.B., G.S.), University of Tennessee,
Knoxville, Tennessee 37996-0845; and Department of Chemistry, College
of Staten Island, City University of New York, Staten Island, New York
10314 (F.R.N.)
We have identified nine oligopeptide transporter (OPT)
orthologs (AtOPT1 to AtOPT9) in
Arabidopsis. These proteins show significant sequence similarity to
OPTs of Candida albicans (CaOpt1p),
Schizosaccharomyces pombe (Isp4p), and
Saccharomyces cerevisiae (Opt1p and Opt2p). Hydrophilicity plots of the OPTs suggest that they are integral membrane proteins with 12 to 14 transmembrane domains. Sequence comparisons showed that the AtOPTs form a distinct subfamily when compared with the fungal OPTs. Two highly conserved motifs (NPG and
KIPPR) were found among all OPT members. The identification of multiple
OPTs in Arabidopsis suggests that they may play different functional
roles. This idea is supported by the fact that AtOPTs have a distinct, tissue-specific expression pattern. The cDNAs encoding
seven of the AtOPTs were cloned into a yeast vector under the control
of a constitutive promoter. AtOPT4 expressed in S. cerevisiae mediated the uptake of KLG-[3H]L.
Similarly, expression of five of the seven AtOPT proteins expressed in
yeast conferred the ability to uptake tetra- and pentapeptides as
measured by growth. This study provides new evidence for multiple
peptide transporter systems in Arabidopsis, suggesting an important
physiological role for small peptides in plants.
1
This work was supported by the U.S. Department
of Agriculture (grant no. 99-35304-8194).
2
Present address: Carnegie Institution of Washington, 260 Panama Street, Stanford, CA 94305-1297.
*
Corresponding author; e-mail gstacey{at}utk.edu; fax 865-974-4007.
© 2002 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
S. Pike, A. Patel, G. Stacey, and W. Gassmann
Arabidopsis OPT6 is an Oligopeptide Transporter with Exceptionally Broad Substrate Specificity
Plant Cell Physiol.,
November 1, 2009;
50(11):
1923 - 1932.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Ueno, N. Yamaji, and J. F. Ma
Further characterization of ferric--phytosiderophore transporters ZmYS1 and HvYS1 in maize and barley
J. Exp. Bot.,
August 1, 2009;
60(12):
3513 - 3520.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. R. Aluru, J. Zola, A. Foudree, and S. R. Rodermel
Chloroplast Photooxidation-Induced Transcriptome Reprogramming in Arabidopsis immutans White Leaf Sectors
Plant Physiology,
June 1, 2009;
150(2):
904 - 923.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. W. Vasconcelos, G. W. Li, M. A. Lubkowitz, and M. A. Grusak
Characterization of the PT Clade of Oligopeptide Transporters in Rice
The Plant Genome,
November 1, 2008;
1(2):
77 - 88.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Tang, A. Hou, M. Babu, V. Nguyen, L. Hurtado, Q. Lu, J. C. Reyes, A. Wang, W. A. Keller, J. J. Harada, et al.
The Arabidopsis BRAHMA Chromatin-Remodeling ATPase Is Involved in Repression of Seed Maturation Genes in Leaves
Plant Physiology,
July 1, 2008;
147(3):
1143 - 1157.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. G. Stacey, A. Patel, W. E. McClain, M. Mathieu, M. Remley, E. E. Rogers, W. Gassmann, D. G. Blevins, and G. Stacey
The Arabidopsis AtOPT3 Protein Functions in Metal Homeostasis and Movement of Iron to Developing Seeds
Plant Physiology,
February 1, 2008;
146(2):
589 - 601.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Wiles, H. Cai, F. Naider, and J. M. Becker
Nutrient regulation of oligopeptide transport in Saccharomyces cerevisiae.
Microbiology,
October 1, 2006;
152(Pt 10):
3133 - 3145.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. M. WATERWORTH and C. M. BRAY
Enigma Variations for Peptides and Their Transporters in Higher Plants
Ann. Bot.,
July 1, 2006;
98(1):
1 - 8.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Chen, E. A. Komives, and J. I. Schroeder
An Improved Grafting Technique for Mature Arabidopsis Plants Demonstrates Long-Distance Shoot-to-Root Transport of Phytochelatins in Arabidopsis
Plant Physiology,
May 1, 2006;
141(1):
108 - 120.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Cagnac, A. Bourbouloux, D. Chakrabarty, M.-Y. Zhang, and S. Delrot
AtOPT6 Transports Glutathione Derivatives and Is Induced by Primisulfuron
Plant Physiology,
July 1, 2004;
135(3):
1378 - 1387.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-Y. Zhang, A. Bourbouloux, O. Cagnac, C. V. Srikanth, D. Rentsch, A. K. Bachhawat, and S. Delrot
A Novel Family of Transporters Mediating the Transport of Glutathione Derivatives in Plants
Plant Physiology,
January 1, 2004;
134(1):
482 - 491.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Wintz, T. Fox, Y.-Y. Wu, V. Feng, W. Chen, H.-S. Chang, T. Zhu, and C. Vulpe
Expression Profiles of Arabidopsis thaliana in Mineral Deficiencies Reveal Novel Transporters Involved in Metal Homeostasis
J. Biol. Chem.,
November 28, 2003;
278(48):
47644 - 47653.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Miyake, H. Hiraishi, H. Sammoto, and B.-I. Ono
Involvement of the VDE Homing Endonuclease and Rapamycin in Regulation of the Saccharomyces cerevisiae GSH11 Gene Encoding the High Affinity Glutathione Transporter
J. Biol. Chem.,
October 10, 2003;
278(41):
39632 - 39636.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. G. Stacey, S. Koh, J. Becker, and G. Stacey
AtOPT3, a Member of the Oligopeptide Transporter Family, Is Essential for Embryo Development in Arabidopsis
PLANT CELL,
November 1, 2002;
14(11):
2799 - 2811.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
