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Plant Physiol, January 2002, Vol. 128, pp. 223-235
Differential Regulation of mRNA Levels of Acyl Carrier Protein
Isoforms in Arabidopsis1
Gustavo
Bonaventure and
John B.
Ohlrogge*
Genetics Program (G.B.) and Department of Plant Biology (J.B.O.),
Michigan State University, East Lansing, Michigan 48824
All higher plants express several different acyl carrier
protein (ACP) isoforms in a tissue-specific manner. We provide evidence that expression of mRNA for the most abundant ACP isoform in
Arabidopsis leaves (ACP4) is increased severalfold by light, whereas
mRNA levels for ACP isoforms 2 and 3 are independent of light. The presence of GATA-like motifs in the upstream region of the
Acl1.4 gene (encoding for ACP4) and the similarity in
light-mediated induction to ferredoxin-A mRNA suggests a direct role of
light in Acl1.4 gene activation. Polyribosomal analysis
indicated that light also affects the association of ACP transcripts
with polysomes, similarly to mRNAs encoding ferredoxin-A. ACP2, ACP3,
and ACP4 mRNA levels were also examined in Arabidopsis cell suspension culture and were found to be differentially controlled by metabolic and/or growth derived signals. Comparison of 5'-untranslated regions (UTRs) of ACP mRNAs of diverse plant species revealed two motifs that
have been conserved during evolution, a CTCCGCC box and C-T-rich sequences. Fusions of the 5'-UTR sequences of ACP1 and ACP2 to luciferase and expression in transgenic plants indicated that the ACP1
leader contributes to preferential expression in seeds, whereas the
ACP2 5'-UTR favored expression in roots. The deletion of 58 bp
containing the conserved motifs of the ACP1 5'-UTR resulted in 10- to
20-fold lower gene expression in leaf and seed tissues of transgenic
Arabidopsis plants.
1
This work was supported by the National Science
Foundation (grant no. MCB98-17882) and by the Michigan Agricultural
Experiment Station.
*
Corresponding author; e-mail ohlrogge{at}imsu.edu; fax
517-353-1926.
© 2002 American Society of Plant Physiologists
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