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Plant Physiol, January 2002, Vol. 128, pp. 247-255

AtFXG1, an Arabidopsis Gene Encoding alpha -L-Fucosidase Active against Fucosylated Xyloglucan Oligosaccharides1

Francisco de la Torre, Javier Sampedro, Ignacio Zarra,* and Gloria Revilla

Departamento de Biología Vegetal, Laboratorio de Fisiología Vegetal, Facultad de Biología, Universidad de Santiago de Compostela, E-15782 Santiago de Compostela, Spain

An alpha -L-fucosidase (EC 3.2.1.51) able to release the t-fucosyl residue from the side chain of xyloglucan oligosaccharides has been detected in the leaves of Arabidopsis plants. Moreover, an alpha -L-fucosidase with similar substrate specificity was purified from cabbage (Brassica oleracea) leaves to render a single band on SDS-PAGE. Two peptide sequences were obtained from this protein band, and they were used to identify an Arabidopsis gene coding for an alpha -fucosidase that we propose to call AtFXG1. In addition, an Arabidopsis gene with homology with known alpha -L-fucosidases has been also found, and we proposed to name it as AtFUC1. Both AtFXG1 and ATFUC1 were heterologously expressed in Pichia pastoris cells and the alpha -L-fucosidase activities secreted to the culture medium. The alpha -L-fucosidase encoded by AtFXG1 was active against the oligosaccharides from xyloglucan XXFG as well as against 2'-fucosyl-lactitol but not against p-nitrophenyl-alpha -L-fucopyranoside. However, the AtFUC1 heterologously expressed was active only against 2'-fucosyl-lactitol. Thus, the former must be related to xyloglucan metabolism.

1 This work was supported by the Dirección General de Investigación (Ministerio de Ciencia y Tecnología, Spain; grant no. PB98-0640) and the Xunta de Galicia (grant no. PGIDT00PXI20002PN).

* Corresponding author; e-mail bvzarra{at}usc.es; fax 34-981-596904.

© 2002 American Society of Plant Physiologists


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