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Plant Physiol, January 2002, Vol. 128, pp. 256-270

A Calcium-Dependent Protein Kinase Is Systemically Induced upon Wounding in Tomato Plants1

José Manuel Chico,2 Marcela Raíces,3 María Teresa Téllez-Iñón, and Rita María Ulloa*

Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas, and Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Vuelta de Obligado 2490 2do piso, 1428 Buenos Aires, Argentina

A full-length cDNA clone (LeCDPK1) from tomato (Lycopersicon esculentum) encoding a calcium-dependent protein kinase (CDPK) was isolated by screening a cDNA library from tomato cell cultures exposed to Cladosporium fulvum elicitor preparations. The predicted amino acid sequence of the cDNA reveals a high degree of similarity with other members of the CDPK family. LeCDPK1 has a putative N-terminal myristoylation sequence and presents a possible palmitoylation site. The in vitro translated protein conserves the biochemical properties of a member of the CDPK family. In addition, CDPK activity was detected in soluble and particulate extracts of tomato leaves. Basal levels of LeCDPK1 mRNA were detected by northern-blot analysis in roots, stems, leaves, and flowers of tomato plants. The expression of LeCDPK1 was rapidly and transiently enhanced in detached tomato leaves treated with pathogen elicitors and H2O2. Moreover, when tomato greenhouse plants were subjected to mechanical wounding, a transient increase of LeCDPK1 steady-state mRNA levels was detected locally at the site of the injury and systemically in distant non-wounded leaves. The increase observed in LeCDPK1 mRNA upon wounding correlates with an increase in the amount and in the activity of a soluble CDPK detected in extracts of tomato leaves, suggesting that this kinase is part of physiological plant defense mechanisms against biotic or abiotic attacks.

1 This work was supported by grants from Agencia de Promoción Científica y Tecnológica, Consejo Nacional de Investigaciones Científicas y Técnicas, and the University of Buenos Aires, Argentina.

2 J.M.C. is a Mutis fellow (Spain).

3 M.R. is a fellow of University of Buenos Aires.

* Corresponding author; e-mail rulloa{at}dna.uba.ar; fax 54-11-4786-8578.

© 2002 American Society of Plant Physiologists


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