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Plant Physiol, January 2002, Vol. 128, pp. 30-37
Functional Analysis of Tomato Pti4 in
Arabidopsis1,2
Keqiang
Wu,
Lining
Tian,
Jamie
Hollingworth,
Daniel C.W.
Brown, and
Brian
Miki*
Department of Biology, West Virginia University, Morgantown, West
Virginia 26506-6057 (K.W.); Southern Crop Protection and Food Research
Centre, Agriculture and Agri-Food Canada, London, Ontario, Canada N5V
4T3 (L.T., D.C.W.B.); and Eastern Cereal and Oilseed Research Centre,
Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada K1A 0C6
(J.H., B.M.)
Pti4 is a tomato (Lycopersicon esculentum)
transcription factor that belongs to the ERF (ethylene-responsive
element binding factor) family of proteins. It interacts with the Pto
kinase in tomato, which confers resistance to the Pseudomonas
syringae pv tomato pathogen that causes
bacterial speck disease. To study the function of Pti4, transgenic
Arabidopsis plants were generated that expressed tomato
Pti4 driven by the strong constitutive promoters, cauliflower mosaic virus 35S and tCUP.
Global gene expression analysis by Affimetric GeneChip indicated that
expression of Pti4 in transgenic Arabidopsis plants
induced the expression of GCC box-containing PR genes. We also
demonstrated that Pti4 enhanced GCC box-mediated transcription of a
reporter gene. The data suggests that tomato Pti4 could act as a
transcriptional activator to regulate expression of GCC box-containing
genes. Furthermore, we show that the expression of tomato
Pti4 in transgenic Arabidopsis plants produced a
phenotype similar to that seen in plants treated with ethylene, thus
providing evidence that the Pti4 gene is involved in the
regulation of a subset of ethylene-responsive genes containing the GCC box.
1
This work was supported in part by the Matching
Investment Initiative Program at Agriculture and Agri-Food Canada. This
paper is Eastern Cereal and Oilseed Research Centre contribution
no. 001554.
2
©Minister of Public Works and Government Services
Canada 2002. For the Department of Agriculture and Agri-Food,
Government of Canada.
*
Corresponding author; e-mail mikib{at}em.agr.ca; fax
613-759- 1701.
© 2002 American Society of Plant Physiologists
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