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Plant Physiol, January 2002, Vol. 128, pp. 84-94
The Arabidopsis Xylem Peptidase XCP1 Is a Tracheary Element
Vacuolar Protein That May Be a Papain Ortholog1
Vanessa
Funk,
Boonthida
Kositsup,2
Chengsong
Zhao, and
Eric P.
Beers*
Department of Horticulture, Virginia Polytechnic Institute and
State University, Blacksburg, Virginia 24061
XCP1 is a xylem-specific papain-like cysteine peptidase in
Arabidopsis. To determine whether XCP1 could be involved in tracheary element autolysis, promoter activity and localization of XCP1 were
investigated using XCP1 promoter- -glucuronidase
fusions and immunofluorescence confocal microscopy. A tracheary element expression pattern was detected for XCP1. Results from
confocal microscopy and biochemical subcellular fractionation indicated that XCP1 was localized in the vacuole. Ectopic expression of XCP1 resulted in a reduction in plant size in some lines
and early leaf senescence, as indicated by early loss of leaf
chlorophyll. Reduced plant size was correlated with higher levels of
XCP1, as shown by immunoblot and peptidase activity gel analyses. The XCP1 prodomain exhibits exceptionally high similarity (greater than
80%) to the prodomains of papain and other papain-like enzymes isolated from papaya (Carica papaya) laticifers when
compared with all other reported papain-like enzymes. The potential for XCP1 and papain to perform common functions as catalysts of autolytic processing following cell death due to programmed suicide or to wounding is discussed.
1
This research was supported by the U.S.
Department of Agriculture-National Research Initiative Competitive
Grants Program (project no. 9801401).
2
Present address: Department of Botany, Faculty of
Science, Chulalong University, Phyathai Road, Patumwan, Bangkok 10330, Thailand.
*
Corresponding author; e-mail ebeers{at}vt.edu; fax
540-231-3083.
© 2002 American Society of Plant Physiologists
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