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Plant Physiol, February 2002, Vol. 128, pp. 379-387
Sodium Fluxes through Nonselective Cation Channels in
the Plasma Membrane of Protoplasts from Arabidopsis
Roots1
Vadim
Demidchik and
Mark
Tester*
Department of Plant Sciences, University of Cambridge, Cambridge,
CB2 3EA United Kingdom
The aim of the present work was to characterize Na+
currents through nonselective cation channels (NSCCs) in protoplasts
derived from root cells of Arabidopsis. The procedure of the protoplast isolation was modified to increase the stability of Arabidopsis root
protoplasts in low external Ca2+ by digesting tissue in
elevated Ca2+. Experiments in whole-cell and outside-out
modes were carried out. We found that Na+ currents in
Arabidopsis root protoplasts were mediated by cation channels that were
insensitive to externally applied
tetraethylammonium+ and verapamil, had no
time-dependent activation (permanently opened or completely activated
within 1-2 ms), were voltage independent, and were weakly selective
for monovalent cations. The selectivity sequence was as follows:
K+ (1.49) > NH4+ (1.24) > Rb+ (1.15)  Cs+ (1.10) Na+ (1.00) > Li+ (0.73) > tetraethylammonium+ (0.47). Arabidopsis root NSCCs were
blocked by H+ (pK 6.0), Ca2+
(K1/2 0.1 mM), Ba2+,
Zn2+, La3+, Gd3+, quinine, and the
His modifier diethylpyrocarbonate. They were insensitive to most
organic blockers (nifedipine, verapamil, flufenamate, and amiloride)
and to the SH-group modifier p-chloromercuriphenyl sulfonic acid. Voltage-insensitive, Ca2+-sensitive single
channels were also resolved. Properties of Arabidopsis root NSCCs are
discussed and compared with characteristics of similar conductances
studied previously in plants and animals. It is suggested that NSCCs
present a distinct group of plant ion channels, mediating toxic
Na+ influx to the cell and probably having other important
roles in physiological processes of plants.
1
This work was supported by the NATO/Royal
Society Fellowship (grants to M.T. and V.D.).
*
Corresponding author: e-mail mat10{at}cam.ac.uk; fax
44-1223-333953.
© 2002 American Society of Plant Physiologists
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