Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiol, February 2002, Vol. 128, pp. 379-387

Sodium Fluxes through Nonselective Cation Channels in the Plasma Membrane of Protoplasts from Arabidopsis Roots1

Vadim Demidchik and Mark Tester*

Department of Plant Sciences, University of Cambridge, Cambridge, CB2 3EA United Kingdom

The aim of the present work was to characterize Na+ currents through nonselective cation channels (NSCCs) in protoplasts derived from root cells of Arabidopsis. The procedure of the protoplast isolation was modified to increase the stability of Arabidopsis root protoplasts in low external Ca2+ by digesting tissue in elevated Ca2+. Experiments in whole-cell and outside-out modes were carried out. We found that Na+ currents in Arabidopsis root protoplasts were mediated by cation channels that were insensitive to externally applied tetraethylammonium+ and verapamil, had no time-dependent activation (permanently opened or completely activated within 1-2 ms), were voltage independent, and were weakly selective for monovalent cations. The selectivity sequence was as follows: K+ (1.49) > NH4+ (1.24) > Rb+ (1.15) approx  Cs+ (1.10) approx  Na+ (1.00) > Li+ (0.73) > tetraethylammonium+ (0.47). Arabidopsis root NSCCs were blocked by H+ (pK approx  6.0), Ca2+ (K1/2 approx  0.1 mM), Ba2+, Zn2+, La3+, Gd3+, quinine, and the His modifier diethylpyrocarbonate. They were insensitive to most organic blockers (nifedipine, verapamil, flufenamate, and amiloride) and to the SH-group modifier p-chloromercuriphenyl sulfonic acid. Voltage-insensitive, Ca2+-sensitive single channels were also resolved. Properties of Arabidopsis root NSCCs are discussed and compared with characteristics of similar conductances studied previously in plants and animals. It is suggested that NSCCs present a distinct group of plant ion channels, mediating toxic Na+ influx to the cell and probably having other important roles in physiological processes of plants.

1 This work was supported by the NATO/Royal Society Fellowship (grants to M.T. and V.D.).

* Corresponding author: e-mail mat10{at}cam.ac.uk; fax 44-1223-333953.

© 2002 American Society of Plant Physiologists


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