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Plant Physiol, February 2002, Vol. 128, pp. 411-417
Inactivation of the Phloem-Specific Dof Zinc Finger Gene
DAG1 Affects Response to Light and Integrity of the Testa
of Arabidopsis Seeds1
Maura
Papi,
Sabrina
Sabatini,2
Maria Maddalena
Altamura,
Lars
Hennig,3
Eberhard
Schäfer,
Paolo
Costantino,* and
Paola
Vittorioso
Istituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di
Genetica e Biologia Molecolare (M.P., S.S., P.C., P.V.) and
Dipartimento di Biologia Vegetale (M.M.A.), Università La
Sapienza, Piazzale Aldo Moro 5, 00185 Rome, Italy; and Institut
für Biologie II, Universität Freiburg,
Schänzlestrasse 1, 79104 Freiburg, Germany (L.H., E.S.)
We show here that seeds from the knockout mutant of the
Arabidopsis DAG1 gene encoding a Dof zinc finger
transcription factor have an altered response to red and far-red light.
Mutant dag1 seeds are induced to germinate by much lower
red light fluence rates, and germination reaches more quickly a point
where it is independent of phytochrome signaling. Moreover, although
microscopic analysis reveals no obvious structural alterations in the
seed coat (testa) of dag1 seeds, staining assays with
different dyes point to an abnormal fragility of the testa. By
extensive in situ mRNA hybridization analysis we show here that the
gene, which is not expressed in the embryo, is specifically expressed
in the phloem of all organs of the mother plant.
1
This work was partially supported by an EC BIO5
contract (Regulatory Gene Initiative in Arabidopsis), by the Ministero
dell' Istruzione, dell' Universita' e della Ricerca and Consiglio
Nazionale delle Ricerche (grant to P.C.), and by Ministero per le
Politiche Agricole (grant to P.C. and M.M.A.). P.V. was the recipient
of a European Molecular Biology Organization short-term fellowship in
Freiburg for the photobiology experiments.
2
Present address: Department of Molecular
Cell Biology, Utrecht University, Padualaan 8 Utrecht, The Netherlands.
3
Present address: ETH Zurich, Institute of Plant
Sciences, ETH Zentrum, LFW E47, Universitätstrasse 2, CH-8092
Zurich, Switzerland.
*
Corresponding author; e-mail paolo.costantino{at}uniroma1.it; fax
39-06-4440812.
© 2002 American Society of Plant Physiologists
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