Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (26)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Quinn, J. M.
Right arrow Articles by Merchant, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Quinn, J. M.
Right arrow Articles by Merchant, S.
Agricola
Right arrow Articles by Quinn, J. M.
Right arrow Articles by Merchant, S.

Plant Physiol, February 2002, Vol. 128, pp. 463-471

Oxygen Deficiency Responsive Gene Expression in Chlamydomonas reinhardtii through a Copper-Sensing Signal Transduction Pathway1

Jeanette M. Quinn, Mats Eriksson,2 Jeffrey L. Moseley, and Sabeeha Merchant*

Department of Chemistry and Biochemistry (J.M.Q., M.E., J.L.M., S.M.) and Molecular Biology Institute (J.M., S.M.), University of California, Los Angeles, California 90095-1569

Chlamydomonas reinhardtii activates Cpx1, Cyc6, and Crd1, encoding, respectively, coproporphyrinogen oxidase, cytochrome c6, and a novel di-iron enzyme when transferred to oxygen-deficient growth conditions. This response is physiologically relevant because C. reinhardtii experiences these growth conditions routinely, and furthermore, one of the target genes, Crd1, is functionally required for normal growth under oxygen-depleted conditions. The same genes are activated also in response to copper-deficiency through copper-response elements that function as target sites for a transcriptional activator. The core of the copper-response element, GTAC, is required also for the hypoxic response, as is a trans-acting locus, CRR1. Mercuric ions, which antagonize the copper-deficiency response, also antagonize the oxygen-deficiency response of these target genes. Taken together, these observations suggest that the oxygen- and copper-deficiency responses share signal transduction components. Nevertheless, whereas the copper-response element is sufficient for the nutritional copper response, the oxygen-deficiency response requires, in addition, a second cis-element, indicating that the response to oxygen depletion is not identical to the nutritional copper response. The distinction between the two responses is also supported by comparative analysis of the response of the target genes, Cyc6, Cpx1, and Crd1, to copper versus oxygen deficiency. A Crr1-independent pathway for Hyd1 expression in oxygen-depleted C. reinhardtii demonstrates the existence of multiple oxygen/redox-responsive circuits in this model organism.

1 This work was supported by the National Institutes of Health (grant no. GM42143). M.E. was supported, in part, by a European Molecular Biology Organization Long-Term Fellowship, and J.L.M., was supported, in part, by the Molecular Biology Ph.D. program and a Dissertation Year Fellowship from the Graduate Division of the University of California (Los Angeles).

2 Present address: Department of Plant Physiology, Umeå University, S-901 87 Umeå, Sweden.

* Corresponding author; e-mail merchant{at}chem.ucla.edu; fax 310-206-1035.

© 2002 American Society of Plant Physiologists


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
A. M. Terauchi, S.-F. Lu, M. Zaffagnini, S. Tappa, M. Hirasawa, J. N. Tripathy, D. B. Knaff, P. J. Farmer, S. D. Lemaire, T. Hase, et al.
Pattern of Expression and Substrate Specificity of Chloroplast Ferredoxins from Chlamydomonas reinhardtii
J. Biol. Chem., September 18, 2009; 284(38): 25867 - 25878.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Dubini, F. Mus, M. Seibert, A. R. Grossman, and M. C. Posewitz
Flexibility in Anaerobic Metabolism as Revealed in a Mutant of Chlamydomonas reinhardtii Lacking Hydrogenase Activity
J. Biol. Chem., March 13, 2009; 284(11): 7201 - 7213.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. D. Page, J. Kropat, P. P. Hamel, and S. S. Merchant
Two Chlamydomonas CTR Copper Transporters with a Novel Cys-Met Motif Are Localized to the Plasma Membrane and Function in Copper Assimilation
PLANT CELL, March 1, 2009; 21(3): 928 - 943.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Surzycki, L. Cournac, G. Peltier, and J.-D. Rochaix
Potential for hydrogen production with inducible chloroplast gene expression in Chlamydomonas
PNAS, October 30, 2007; 104(44): 17548 - 17553.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Mus, A. Dubini, M. Seibert, M. C. Posewitz, and A. R. Grossman
Anaerobic Acclimation in Chlamydomonas reinhardtii: ANOXIC GENE EXPRESSION, HYDROGENASE INDUCTION, AND METABOLIC PATHWAYS
J. Biol. Chem., August 31, 2007; 282(35): 25475 - 25486.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Ouchane, M. Picaud, P. Therizols, F. Reiss-Husson, and C. Astier
Global Regulation of Photosynthesis and Respiration by FnrL: THE FIRST TWO TARGETS IN THE TETRAPYRROLE PATHWAY
J. Biol. Chem., March 9, 2007; 282(10): 7690 - 7699.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Kropat, S. Tottey, R. P. Birkenbihl, N. Depege, P. Huijser, and S. Merchant
A regulator of nutritional copper signaling in Chlamydomonas is an SBP domain protein that recognizes the GTAC core of copper response element
PNAS, December 20, 2005; 102(51): 18730 - 18735.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
J. BAILEY-SERRES and R. CHANG
Sensing and Signalling in Response to Oxygen Deprivation in Plants and Other Organisms
Ann. Bot., September 1, 2005; 96(4): 507 - 518.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. Eriksson, J. L. Moseley, S. Tottey, J. A. del Campo, J. Quinn, Y. Kim, and S. Merchant
Genetic Dissection of Nutritional Copper Signaling in Chlamydomonas Distinguishes Regulatory and Target Genes
Genetics, October 1, 2004; 168(2): 795 - 807.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Ouchane, A.-S. Steunou, M. Picaud, and C. Astier
Aerobic and Anaerobic Mg-Protoporphyrin Monomethyl Ester Cyclases in Purple Bacteria: A STRATEGY ADOPTED TO BYPASS THE REPRESSIVE OXYGEN CONTROL SYSTEM
J. Biol. Chem., February 20, 2004; 279(8): 6385 - 6394.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A.-L. Paul, A. C. Schuerger, M. P. Popp, J. T. Richards, M. S. Manak, and R. J. Ferl
Hypobaric Biology: Arabidopsis Gene Expression at Low Atmospheric Pressure
Plant Physiology, January 1, 2004; 134(1): 215 - 223.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
J. M. Quinn, J. Kropat, and S. Merchant
Copper Response Element and Crr1-Dependent Ni2+-Responsive Promoter for Induced, Reversible Gene Expression in Chlamydomonas reinhardtii
Eukaryot. Cell, October 1, 2003; 2(5): 995 - 1002.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. L. Moseley, M. D. Page, N. P. Alder, M. Eriksson, J. Quinn, F. Soto, S. M. Theg, M. Hippler, and S. Merchant
Reciprocal Expression of Two Candidate Di-Iron Enzymes Affecting Photosystem I and Light-Harvesting Complex Accumulation
PLANT CELL, March 1, 2002; 14(3): 673 - 688.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2002 by the American Society of Plant Biologists