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Plant Physiol, February 2002, Vol. 128, pp. 472-481

Arabidopsis Seedling Growth, Storage Lipid Mobilization, and Photosynthetic Gene Expression Are Regulated by Carbon:Nitrogen Availability1

Thomas Martin,2 Oliver Oswald,3 and Ian A. Graham*

Plant Molecular Science Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, United Kingdom (T.M., O.O.); and Centre for Novel Agricultural Products, Department of Biology, University of York, P.O. Box 373, York YO10 5YW, United Kingdom (I.A.G.)

The objective of the current work was to establish the degree to which the effects of carbon and nitrogen availability on Arabidopsis seedling growth and development are due to these nutrients acting independently or together. Growth of seedlings on low (0.1 mM) nitrogen results in a significant reduction of seedling and cotyledon size, fresh weight, chlorophyll, and anthocyanin content but a slight increase in endogenous sugars. The addition of 100 mM sucrose (Suc) to the nitrogen-depleted growth media results in a further reduction in cotyledon size and chlorophyll content and an overall increase in anthocyanins and endogenous sugars. Storage lipid breakdown is almost completely blocked in seedlings grown on low nitrogen and 100 mM Suc and is significantly inhibited when seedlings are grown on either low nitrogen or high Suc. Carbohydrate repression of photosynthetic gene expression can only be observed under low nitrogen conditions. Low (0.1 mM) nitrogen in the absence of exogenous carbohydrate results in a significant decrease in chlorophyll a/b-binding protein and ribulose bisphosphate carboxylase small subunit gene transcript levels. Thus, carbon to nitrogen ratio rather than carbohydrate status alone appears to play the predominant role in regulating various aspects of seedling growth including storage reserve mobilization and photosynthetic gene expression.

1 This work was supported by the Biotechnology and Biological Science Research Council (grant no. PO5195) and the European Community Framework program IV (contract no. BI04 CT960311). O.O. was funded through a University of Glasgow, Institute of Biomedical and Life Sciences PhD studentship.

2 Present address: University of Cambridge, Department of Plant Sciences, Downing Street, Cambridge, CB2 3EA, UK.

3 Present address: BASF Plant Science GmbH, BPS-A30, D-67056, Ludwigshafen, Germany.

* Corresponding author; e-mail iag1{at}york.ac.uk; fax 44-1904-43-43-00.

© 2002 American Society of Plant Physiologists


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