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Plant Physiol, February 2002, Vol. 128, pp. 512-522
The Plastidic Pentose Phosphate Translocator Represents a Link
between the Cytosolic and the Plastidic Pentose Phosphate Pathways in
Plants1
Michael
Eicks,
Verónica
Maurino,
Silke
Knappe,
Ulf-Ingo
Flügge, and
Karsten
Fischer*
Botanisches Institut der Universität zu Köln, Lehrstuhl
II, Gyrhofstrasse 15, D-50931 Cologne, Germany
Plastids are the site of the reductive and the oxidative
pentose phosphate pathways, which both generate pentose phosphates as
intermediates. A plastidic transporter from Arabidopsis has been
identified that is able to transport, in exchange with inorganic phosphate or triose phosphates, xylulose 5-phosphate (Xul-5-P) and, to
a lesser extent, also ribulose 5-phosphate, but does not accept ribose
5-phosphate or hexose phosphates as substrates. Under physiological
conditions, Xul-5-P would be the preferred substrate. Therefore, the
translocator was named Xul-5-P/phosphate translocator (XPT). The XPT
shares only approximately 35% to 40% sequence identity with members
of both the triose phosphate translocator and the
phosphoenolpyruvate/phosphate translocator classes, but a higher identity of approximately 50% to glucose
6-phosphate/phosphate translocators. Therefore, it represents a fourth
group of plastidic phosphate translocators. Database analysis revealed
that plant cells contain, in addition to enzymes of the oxidative
branch of the oxidative pentose phosphate pathway, ribose 5-phosphate isomerase and ribulose 5-phosphate epimerase in both the cytosol and
the plastids, whereas the transketolase and transaldolase converting
the produced pentose phosphates to triose phosphates and hexose
phosphates are probably solely confined to plastids. It is assumed that
the XPT function is to provide the plastidic pentose phosphate pathways
with cytosolic carbon skeletons in the form of Xul-5-P, especially
under conditions of a high demand for intermediates of the cycles.
1
This work was supported by the Deutsche
Forschungsgemeinschaft and by the Alexander von Humboldt-Foundation
(postdoctorate fellowship to V.M.).
*
Corresponding author; e-mail karsten.fischer{at}uni-koeln.de; fax
49-221-470-5039.
© 2002 American Society of Plant Physiologists
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