|
Plant Physiol, February 2002, Vol. 128, pp. 544-551
Overexpression of a 9-cis-Epoxycarotenoid
Dioxygenase Gene in Nicotiana plumbaginifolia Increases
Abscisic Acid and Phaseic Acid Levels and Enhances Drought
Tolerance1
Xiaoqiong
Qin and
Jan A.D.
Zeevaart*
Departments of Energy-Plant Research Laboratory (X.Q., J.A.D.Z.)
and Plant Biology (J.A.D.Z.), Michigan State University, East Lansing,
Michigan 48824-1312
The plant hormone abscisic acid (ABA) plays important roles in seed
maturation and dormancy and in adaptation to a variety of environmental
stresses. An effort to engineer plants with elevated ABA levels and
subsequent stress tolerance is focused on the genetic manipulation of
the cleavage reaction. It has been shown in bean (Phaseolus
vulgaris) that the gene encoding the cleavage enzyme (PvNCED1) is up-regulated by water stress, preceding
accumulation of ABA. Transgenic wild tobacco (Nicotiana
plumbaginifolia Viv.) plants were produced that overexpress the
PvNCED1 gene either constitutively or in an inducible
manner. The constitutive expression of PvNCED1 resulted
in an increase in ABA and its catabolite, phaseic acid (PA). When the
PvNCED1 gene was driven by the dexamethasone (DEX)-inducible promoter, a transient induction of
PvNCED1 message and accumulation of ABA and PA were
observed in different lines after application of DEX. Accumulation of
ABA started to level off after 6 h, whereas the PA level continued
to increase. In the presence of DEX, seeds from homozygous transgenic
line TN1 showed a 4-d delay in germination. After spraying with DEX,
the detached leaves from line TN1 had a drastic decrease in their water
loss relative to control leaves. These plants also showed a marked
increase in their tolerance to drought stress. These results indicate
that it is possible to manipulate ABA levels in plants by
overexpressing the key regulatory gene in ABA biosynthesis and that
stress tolerance can be improved by increasing ABA levels.
1
This work was supported by the National Science
Foundation (grant no. IBN-9982758) and by the U.S. Department of
Energy (grant no. DE-FG02-91ER20021).
*
Corresponding author; e-mail zeevaart{at}msu.edu; fax
517-353-9168.
© 2002 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
A. Ben Hassine, M. E. Ghanem, S. Bouzid, and S. Lutts
Abscisic acid has contrasting effects on salt excretion and polyamine concentrations of an inland and a coastal population of the Mediterranean xero-halophyte species Atriplex halimus
Ann. Bot.,
October 1, 2009;
104(5):
925 - 936.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Wan, R. Griffiths, J. Ying, P. McCourt, and Y. Huang
Development of Drought-Tolerant Canola (Brassica napus L.) through Genetic Modulation of ABA-mediated Stomatal Responses
Crop Sci.,
August 7, 2009;
49(5):
1539 - 1554.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B.-Z. Xiao, X. Chen, C.-B. Xiang, N. Tang, Q.-F. Zhang, and L.-Z. Xiong
Evaluation of Seven Function-Known Candidate Genes for their Effects on Improving Drought Resistance of Transgenic Rice under Field Conditions
Mol Plant,
January 1, 2009;
2(1):
73 - 83.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Endo, Y. Sawada, H. Takahashi, M. Okamoto, K. Ikegami, H. Koiwai, M. Seo, T. Toyomasu, W. Mitsuhashi, K. Shinozaki, et al.
Drought Induction of Arabidopsis 9-cis-Epoxycarotenoid Dioxygenase Occurs in Vascular Parenchyma Cells
Plant Physiology,
August 1, 2008;
147(4):
1984 - 1993.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Welsch, F. Wust, C. Bar, S. Al-Babili, and P. Beyer
A Third Phytoene Synthase Is Devoted to Abiotic Stress-Induced Abscisic Acid Formation in Rice and Defines Functional Diversification of Phytoene Synthase Genes
Plant Physiology,
May 1, 2008;
147(1):
367 - 380.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. T. Vogel, B.-C. Tan, D. R. McCarty, and H. J. Klee
The Carotenoid Cleavage Dioxygenase 1 Enzyme Has Broad Substrate Specificity, Cleaving Multiple Carotenoids at Two Different Bond Positions
J. Biol. Chem.,
April 25, 2008;
283(17):
11364 - 11373.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Toh, A. Imamura, A. Watanabe, K. Nakabayashi, M. Okamoto, Y. Jikumaru, A. Hanada, Y. Aso, K. Ishiyama, N. Tamura, et al.
High Temperature-Induced Abscisic Acid Biosynthesis and Its Role in the Inhibition of Gibberellin Action in Arabidopsis Seeds
Plant Physiology,
March 1, 2008;
146(3):
1368 - 1385.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. Thompson, J. Andrews, B. J. Mulholland, J. M.T. McKee, H. W. Hilton, J. S. Horridge, G. D. Farquhar, R. C. Smeeton, I. R.A. Smillie, C. R. Black, et al.
Overproduction of Abscisic Acid in Tomato Increases Transpiration Efficiency and Root Hydraulic Conductivity and Influences Leaf Expansion
Plant Physiology,
April 1, 2007;
143(4):
1905 - 1917.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P.-C. Lin, S.-G. Hwang, A. Endo, M. Okamoto, T. Koshiba, and W.-H. Cheng
Ectopic Expression of ABSCISIC ACID 2/GLUCOSE INSENSITIVE 1 in Arabidopsis Promotes Seed Dormancy and Stress Tolerance
Plant Physiology,
February 1, 2007;
143(2):
745 - 758.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Saez, N. Robert, M. H. Maktabi, J. I. Schroeder, R. Serrano, and P. L. Rodriguez
Enhancement of Abscisic Acid Sensitivity and Reduction of Water Consumption in Arabidopsis by Combined Inactivation of the Protein Phosphatases Type 2C ABI1 and HAB1
Plant Physiology,
August 1, 2006;
141(4):
1389 - 1399.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P.-A. Vidi, M. Kanwischer, S. Baginsky, J. R. Austin, G. Csucs, P. Dormann, F. Kessler, and C. Brehelin
Tocopherol Cyclase (VTE1) Localization and Vitamin E Accumulation in Chloroplast Plastoglobule Lipoprotein Particles
J. Biol. Chem.,
April 21, 2006;
281(16):
11225 - 11234.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-J. Rodrigo, B. Alquezar, and L. Zacarias
Cloning and characterization of two 9-cis-epoxycarotenoid dioxygenase genes, differentially regulated during fruit maturation and under stress conditions, from orange (Citrus sinensis L. Osbeck)
J. Exp. Bot.,
February 1, 2006;
57(3):
633 - 643.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. E. Verslues and E. A. Bray
Role of abscisic acid (ABA) and Arabidopsis thaliana ABA-insensitive loci in low water potential-induced ABA and proline accumulation
J. Exp. Bot.,
January 1, 2006;
57(1):
201 - 212.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. D. Cameron, M. A. Teece, and L. B. Smart
Increased Accumulation of Cuticular Wax and Expression of Lipid Transfer Protein in Response to Periodic Drying Events in Leaves of Tree Tobacco
Plant Physiology,
January 1, 2006;
140(1):
176 - 183.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Chen, F. Ji, H. Xie, J. Liang, and J. Zhang
The Regulator of G-Protein Signaling Proteins Involved in Sugar and Abscisic Acid Signaling in Arabidopsis Seed Germination
Plant Physiology,
January 1, 2006;
140(1):
302 - 310.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. A. Wolucka, A. Goossens, and D. Inze
Methyl jasmonate stimulates the de novo biosynthesis of vitamin C in plant cell suspensions
J. Exp. Bot.,
September 1, 2005;
56(419):
2527 - 2538.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Seo, H. Aoki, H. Koiwai, Y. Kamiya, E. Nambara, and T. Koshiba
Comparative Studies on the Arabidopsis Aldehyde Oxidase (AAO) Gene Family Revealed a Major Role of AAO3 in ABA Biosynthesis in Seeds
Plant Cell Physiol.,
November 15, 2004;
45(11):
1694 - 1703.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Saito, N. Hirai, C. Matsumoto, H. Ohigashi, D. Ohta, K. Sakata, and M. Mizutani
Arabidopsis CYP707As Encode (+)-Abscisic Acid 8'-Hydroxylase, a Key Enzyme in the Oxidative Catabolism of Abscisic Acid
Plant Physiology,
April 1, 2004;
134(4):
1439 - 1449.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Xiong and J.-K. Zhu
Regulation of Abscisic Acid Biosynthesis
Plant Physiology,
September 1, 2003;
133(1):
29 - 36.
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. O. Lindgren, K. G. Stalberg, and A.-S. Hoglund
Seed-Specific Overexpression of an Endogenous Arabidopsis Phytoene Synthase Gene Results in Delayed Germination and Increased Levels of Carotenoids, Chlorophyll, and Abscisic Acid
Plant Physiology,
June 1, 2003;
132(2):
779 - 785.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. H. Schwartz, X. Qin, and J. A.D. Zeevaart
Elucidation of the Indirect Pathway of Abscisic Acid Biosynthesis by Mutants, Genes, and Enzymes
Plant Physiology,
April 1, 2003;
131(4):
1591 - 1601.
[Full Text]
[PDF]
|
|
|
|
|
|
|
W.-H. Cheng, A. Endo, L. Zhou, J. Penney, H.-C. Chen, A. Arroyo, P. Leon, E. Nambara, T. Asami, M. Seo, et al.
A Unique Short-Chain Dehydrogenase/Reductase in Arabidopsis Glucose Signaling and Abscisic Acid Biosynthesis and Functions
PLANT CELL,
November 1, 2002;
14(11):
2723 - 2743.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
