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Plant Physiol, February 2002, Vol. 128, pp. 552-563

Salicylic Acid Has Cell-Specific Effects on Tobacco mosaic virus Replication and Cell-to-Cell Movement1

Alex M. Murphy and John P. Carr*

Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, United Kingdom

Tobacco mosaic virus (TMV) and Cucumber mosaic virus expressing green fluorescent protein (GFP) were used to probe the effects of salicylic acid (SA) on the cell biology of viral infection. Treatment of tobacco with SA restricted TMV.GFP to single-epidermal cell infection sites for at least 6 d post inoculation but did not affect infection sites of Cucumber mosaic virus expressing GFP. Microinjection experiments, using size-specific dextrans, showed that SA cannot inhibit TMV movement by decreasing the plasmodesmatal size exclusion limit. In SA-treated transgenic plants expressing TMV movement protein, TMV.GFP infection sites were larger, but they still consisted overwhelmingly of epidermal cells. TMV replication was strongly inhibited in mesophyll protoplasts isolated from SA-treated nontransgenic tobacco plants. Therefore, it appears that SA has distinct cell type-specific effects on virus replication and movement in the mesophyll and epidermal cell layers, respectively. Thus, SA can have fundamentally different effects on the same pathogen in different cell types.

1 The initial phase of this work was supported by the Biotechnological and Biological Sciences Research Council (grant no. PO3659 to J.P.C.) and subsequently by the Cambridge University Broodbank Fund and a grant from the Royal Society (to A.M.M.).

* Corresponding author; e-mail jpc1005{at}hermes.cam.ac.uk; fax 44-1223-333953.

© 2002 American Society of Plant Physiologists


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