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Plant Physiol, February 2002, Vol. 128, pp. 552-563
Salicylic Acid Has Cell-Specific Effects on Tobacco mosaic
virus Replication and Cell-to-Cell Movement1
Alex M.
Murphy and
John P.
Carr*
Department of Plant Sciences, University of Cambridge, Cambridge
CB2 3EA, United Kingdom
Tobacco mosaic virus (TMV) and Cucumber mosaic
virus expressing green fluorescent protein (GFP) were used to
probe the effects of salicylic acid (SA) on the cell biology of viral
infection. Treatment of tobacco with SA restricted TMV.GFP to
single-epidermal cell infection sites for at least 6 d post
inoculation but did not affect infection sites of Cucumber mosaic
virus expressing GFP. Microinjection experiments, using
size-specific dextrans, showed that SA cannot inhibit TMV movement by
decreasing the plasmodesmatal size exclusion limit. In SA-treated
transgenic plants expressing TMV movement protein, TMV.GFP infection
sites were larger, but they still consisted overwhelmingly of epidermal
cells. TMV replication was strongly inhibited in mesophyll protoplasts
isolated from SA-treated nontransgenic tobacco plants. Therefore, it
appears that SA has distinct cell type-specific effects on virus
replication and movement in the mesophyll and epidermal cell layers,
respectively. Thus, SA can have fundamentally different effects on the
same pathogen in different cell types.
1
The initial phase of this work was supported by
the Biotechnological and Biological Sciences Research Council (grant
no. PO3659 to J.P.C.) and subsequently by the Cambridge University
Broodbank Fund and a grant from the Royal Society (to A.M.M.).
*
Corresponding author; e-mail jpc1005{at}hermes.cam.ac.uk; fax
44-1223-333953.
© 2002 American Society of Plant Physiologists
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