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Plant Physiol, February 2002, Vol. 128, pp. 650-660
Biochemical and Molecular Characterization of a Mutation That
Confers a Decreased Raffinosaccharide and Phytic Acid Phenotype on
Soybean Seeds
William D.
Hitz,*
Thomas J.
Carlson,
Phil S.
Kerr, and
Scott A.
Sebastian
DuPont Crop Genetics, P.O. Box 80402, Wilmington, Delaware
19880-0402 (W.D.H., T.J.C.); Protein Technologies International, 14440 Danforth Drive, St. Louis, Missouri (P.S.K.); and Pioneer Hibred
International, Johnston, Iowa (S.A.S.)
A single, recessive mutation in soybean
(Glycine max L. Merr.), which confers a seed phenotype
of increased inorganic phosphate, decreased phytic acid, and a decrease
in total raffinosaccharides, has been previously disclosed (S.A.
Sebastian, P.S. Kerr, R.W. Pearlstein, W.D. Hitz [2000] Soy in Animal
Nutrition, pp 56-74). The genetic lesion causing the multiple changes
in seed phenotype is a single base change in the third base of the
codon for what is amino acid residue 396 of the mature peptide encoding
a seed-expressed myo-inositol 1-phospate synthase gene.
The base change causes residue 396 to change from lysine to asparagine.
That amino acid change decreases the specific activity of the
seed-expressed myo-inositol 1-phosphate synthase by
about 90%. Radio tracer experiments indicate that the supply of
myo-inositol to the reaction, which converts UDP-galactose and myo-inositol to galactinol is a
controlling factor in the conversion of total carbohydrate into the
raffinosaccharides in both wild-type and mutant lines. That same
decrease in myo-inositol 1-phosphate synthetic capacity
leads to a decreased capacity for the synthesis of
myo-inositol hexaphosphate (phytic acid) and a
concomitant increase in inorganic phosphate.
*
Corresponding author; e-mail
william.d.hitz{at}usa.dupont.com; fax 302-695-9149.
© 2002 American Society of Plant Physiologists
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