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Plant Physiol, February 2002, Vol. 128, pp. 650-660

Biochemical and Molecular Characterization of a Mutation That Confers a Decreased Raffinosaccharide and Phytic Acid Phenotype on Soybean Seeds

William D. Hitz,* Thomas J. Carlson, Phil S. Kerr, and Scott A. Sebastian

DuPont Crop Genetics, P.O. Box 80402, Wilmington, Delaware 19880-0402 (W.D.H., T.J.C.); Protein Technologies International, 14440 Danforth Drive, St. Louis, Missouri (P.S.K.); and Pioneer Hibred International, Johnston, Iowa (S.A.S.)

A single, recessive mutation in soybean (Glycine max L. Merr.), which confers a seed phenotype of increased inorganic phosphate, decreased phytic acid, and a decrease in total raffinosaccharides, has been previously disclosed (S.A. Sebastian, P.S. Kerr, R.W. Pearlstein, W.D. Hitz [2000] Soy in Animal Nutrition, pp 56-74). The genetic lesion causing the multiple changes in seed phenotype is a single base change in the third base of the codon for what is amino acid residue 396 of the mature peptide encoding a seed-expressed myo-inositol 1-phospate synthase gene. The base change causes residue 396 to change from lysine to asparagine. That amino acid change decreases the specific activity of the seed-expressed myo-inositol 1-phosphate synthase by about 90%. Radio tracer experiments indicate that the supply of myo-inositol to the reaction, which converts UDP-galactose and myo-inositol to galactinol is a controlling factor in the conversion of total carbohydrate into the raffinosaccharides in both wild-type and mutant lines. That same decrease in myo-inositol 1-phosphate synthetic capacity leads to a decreased capacity for the synthesis of myo-inositol hexaphosphate (phytic acid) and a concomitant increase in inorganic phosphate.

* Corresponding author; e-mail william.d.hitz{at}usa.dupont.com; fax 302-695-9149.

© 2002 American Society of Plant Physiologists


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