|
Plant Physiol, February 2002, Vol. 128, pp. 682-695
Protection against Heat Stress-Induced Oxidative Damage in
Arabidopsis Involves Calcium, Abscisic Acid, Ethylene, and Salicylic
Acid
Jane
Larkindale* and
Marc R.
Knight
Department of Plant Sciences, University of Oxford, South Parks
Road, Oxford OX1 3RB, United Kingdom
Plants, in common with all organisms, have evolved
mechanisms to cope with the problems caused by high temperatures. We
examined specifically the involvement of calcium, abscisic acid (ABA), ethylene, and salicylic acid (SA) in the protection against
heat-induced oxidative damage in Arabidopsis. Heat caused increased
thiobarbituric acid reactive substance levels (an indicator of
oxidative damage to membranes) and reduced survival. Both effects
required light and were reduced in plants that had acquired
thermotolerance through a mild heat pretreatment. Calcium channel
blockers and calmodulin inhibitors increased these effects of heating
and added calcium reversed them, implying that protection against
heat-induced oxidative damage in Arabidopsis requires calcium and
calmodulin. Similar to calcium, SA, 1-aminocyclopropane-1-carboxylic
acid (a precursor to ethylene), and ABA added to plants protected them
from heat-induced oxidative damage. In addition, the
ethylene-insensitive mutant etr-1, the ABA-insensitive
mutant abi-1, and a transgenic line expressing
nahG (consequently inhibited in SA production) showed increased susceptibility to heat. These data suggest that protection against heat-induced oxidative damage in Arabidopsis also involves ethylene, ABA, and SA. Real time measurements of cytosolic calcium levels during heating in Arabidopsis detected no increases in response
to heat per se, but showed transient elevations in response to recovery
from heating. The magnitude of these calcium peaks was greater in
thermotolerant plants, implying that these calcium signals might play a
role in mediating the effects of acquired thermotolerance. Calcium
channel blockers and calmodulin inhibitors added solely during the
recovery phase suggest that this role for calcium is in protecting
against oxidative damage specifically during/after recovery.
*
Corresponding author; e-mail jane.larkindale{at}plants.ox.ac.uk; fax
44-1865-275074.
© 2002 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
L. Zhang, Y. Li, D. Xing, and C. Gao
Characterization of mitochondrial dynamics and subcellular localization of ROS reveal that HsfA2 alleviates oxidative damage caused by heat stress in Arabidopsis
J. Exp. Bot.,
May 1, 2009;
60(7):
2073 - 2091.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Zhang, R.-G. Zhou, Y.-J. Gao, S.-Z. Zheng, P. Xu, S.-Q. Zhang, and D.-Y. Sun
Molecular and Genetic Evidence for the Key Role of AtCaM3 in Heat-Shock Signal Transduction in Arabidopsis
Plant Physiology,
April 1, 2009;
149(4):
1773 - 1784.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. E. Abreu and S. Munne-Bosch
Salicylic acid deficiency in NahG transgenic lines and sid2 mutants increases seed yield in the annual plant Arabidopsis thaliana
J. Exp. Bot.,
March 1, 2009;
60(4):
1261 - 1271.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A.-R. Mohammed and L. Tarpley
Impact of High Nighttime Temperature on Respiration, Membrane Stability, Antioxidant Capacity, and Yield of Rice Plants
Crop Sci.,
January 1, 2009;
49(1):
313 - 322.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Snyman and M. J. Cronje
Modulation of heat shock factors accompanies salicylic acid-mediated potentiation of Hsp70 in tomato seedlings
J. Exp. Bot.,
May 8, 2008;
(2008)
ern075v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Suzuki, S. Bajad, J. Shuman, V. Shulaev, and R. Mittler
The Transcriptional Co-activator MBF1c Is a Key Regulator of Thermotolerance in Arabidopsis thaliana
J. Biol. Chem.,
April 4, 2008;
283(14):
9269 - 9275.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Larkindale and E. Vierling
Core Genome Responses Involved in Acclimation to High Temperature
Plant Physiology,
February 1, 2008;
146(2):
748 - 761.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Kant, S. Kant, M. Gordon, R. Shaked, and S. Barak
STRESS RESPONSE SUPPRESSOR1 and STRESS RESPONSE SUPPRESSOR2, Two DEAD-Box RNA Helicases That Attenuate Arabidopsis Responses to Multiple Abiotic Stresses
Plant Physiology,
November 1, 2007;
145(3):
814 - 830.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Y. Yoo, K. Miura, J. B. Jin, J. Lee, H. C. Park, D. E. Salt, D.-J. Yun, R. A. Bressan, and P. M. Hasegawa
SIZ1 Small Ubiquitin-Like Modifier E3 Ligase Facilitates Basal Thermotolerance in Arabidopsis Independent of Salicylic Acid
Plant Physiology,
December 1, 2006;
142(4):
1548 - 1558.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Chen and D. R. Gallie
Dehydroascorbate Reductase Affects Leaf Growth, Development, and Function
Plant Physiology,
October 1, 2006;
142(2):
775 - 787.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H.-T. Liu, Y.-Y. Liu, Q.-H. Pan, H.-R. Yang, J.-C. Zhan, and W.-D. Huang
Novel interrelationship between salicylic acid, abscisic acid, and PIP2-specific phospholipase C in heat acclimation-induced thermotolerance in pea leaves
J. Exp. Bot.,
September 1, 2006;
57(12):
3337 - 3347.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Santos, M. Gaspar, A. Caeiro, C. Branco-Price, A. Teixeira, and R. B. Ferreira
Exposure of Lemna minor to Arsenite: Expression Levels of the Components and Intermediates of the Ubiquitin/Proteasome Pathway
Plant Cell Physiol.,
September 1, 2006;
47(9):
1262 - 1273.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. MILLER and R. MITTLER
Could Heat Shock Transcription Factors Function as Hydrogen Peroxide Sensors in Plants?
Ann. Bot.,
August 1, 2006;
98(2):
279 - 288.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Vanderauwera, P. Zimmermann, S. Rombauts, S. Vandenabeele, C. Langebartels, W. Gruissem, D. Inze, and F. Van Breusegem
Genome-Wide Analysis of Hydrogen Peroxide-Regulated Gene Expression in Arabidopsis Reveals a High Light-Induced Transcriptional Cluster Involved in Anthocyanin Biosynthesis
Plant Physiology,
October 1, 2005;
139(2):
806 - 821.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Barrero, P. Piqueras, M. Gonzalez-Guzman, R. Serrano, P. L. Rodriguez, M. R. Ponce, and J. L. Micol
A mutational analysis of the ABA1 gene of Arabidopsis thaliana highlights the involvement of ABA in vegetative development
J. Exp. Bot.,
August 1, 2005;
56(418):
2071 - 2083.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Larkindale, J. D. Hall, M. R. Knight, and E. Vierling
Heat Stress Phenotypes of Arabidopsis Mutants Implicate Multiple Signaling Pathways in the Acquisition of Thermotolerance
Plant Physiology,
June 1, 2005;
138(2):
882 - 897.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. He, Y. Liu, W. Cao, M. Huai, B. Xu, and B. Huang
Effects of Salicylic Acid on Heat Tolerance Associated with Antioxidant Metabolism in Kentucky Bluegrass
Crop Sci.,
May 6, 2005;
45(3):
988 - 995.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Freeman, D. Garcia, D. Kim, A. Hopf, and D. E. Salt
Constitutively Elevated Salicylic Acid Signals Glutathione-Mediated Nickel Tolerance in Thlaspi Nickel Hyperaccumulators
Plant Physiology,
March 1, 2005;
137(3):
1082 - 1091.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Munne-Bosch, J. Penuelas, D. Asensio, and J. Llusia
Airborne Ethylene May Alter Antioxidant Protection and Reduce Tolerance of Holm Oak to Heat and Drought Stress
Plant Physiology,
October 1, 2004;
136(2):
2937 - 2947.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Price, A. Laxmi, S. K. St. Martin, and J.-C. Jang
Global Transcription Profiling Reveals Multiple Sugar Signal Transduction Mechanisms in Arabidopsis
PLANT CELL,
August 1, 2004;
16(8):
2128 - 2150.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. M. Scott, S. M. Clarke, J. E. Wood, and L. A.J. Mur
Salicylate Accumulation Inhibits Growth at Chilling Temperature in Arabidopsis
Plant Physiology,
June 1, 2004;
135(2):
1040 - 1049.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Li, H.-T. Liu, D.-Y. Sun, and R.-G. Zhou
Ca2+ and Calmodulin Modulate DNA-Binding Activity of Maize Heat Shock Transcription Factor in Vitro
Plant Cell Physiol.,
May 15, 2004;
45(5):
627 - 634.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H.-T. Liu, B. Li, Z.-L. Shang, X.-Z. Li, R.-L. Mu, D.-Y. Sun, and R.-G. Zhou
Calmodulin Is Involved in Heat Shock Signal Transduction in Wheat
Plant Physiology,
July 1, 2003;
132(3):
1186 - 1195.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-W. Hong, U. Lee, and E. Vierling
Arabidopsis hot Mutants Define Multiple Functions Required for Acclimation to High Temperatures
Plant Physiology,
June 1, 2003;
132(2):
757 - 767.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Bouche, A. Fait, D. Bouchez, S. G. Moller, and H. Fromm
Mitochondrial succinic-semialdehyde dehydrogenase of the {gamma}-aminobutyrate shunt is required to restrict levels of reactive oxygen intermediates in plants
PNAS,
May 27, 2003;
100(11):
6843 - 6848.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Yang and B. W. Poovaiah
A Calmodulin-binding/CGCG Box DNA-binding Protein Family Involved in Multiple Signaling Pathways in Plants
J. Biol. Chem.,
November 15, 2002;
277(47):
45049 - 45058.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
