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Plant Physiol, February 2002, Vol. 128, pp. 726-733
Flavone Glucoside Uptake into Barley Mesophyll and Arabidopsis
Cell Culture Vacuoles. Energization Occurs by H+-Antiport
and ATP-Binding Cassette-Type Mechanisms1
Nathalie
Frangne,2
Thomas
Eggmann,
Carsten
Koblischke,
Gottfried
Weissenböck,
Enrico
Martinoia, and
Markus
Klein*
Institut de Botanique, Laboratoire de Physiologie
Végétale, Université de Neuchâtel, Rue Emile
Argand 13, CH-2007 Neuchâtel, Switzerland (N.F., T.E., E.M.,
M.K.); and Botanisches Institut der Universität zu Köln,
Gyrhofstrasse 51, D-50931 Cologne, Germany (C.K., G.W.)
In many cases, secondary plant products accumulate in the large
central vacuole of plant cells. However, the mechanisms involved in the
transport of secondary compounds are only poorly understood. Here, we
demonstrate that the transport mechanisms for the major barley
(Hordeum vulgare) flavonoid saponarin (apigenin
6-C-glucosyl-7-O-glucoside) are different
in various plant species: Uptake into barley vacuoles occurs via a
proton antiport and is competitively inhibited by isovitexin (apigenin
6-C-glucoside), suggesting that both flavone glucosides
are recognized by the same transporter. In contrast, the transport into
vacuoles from Arabidopsis, which does not synthesize flavone
glucosides, displays typical characteristics of ATP-binding cassette
transporters. Transport of saponarin into vacuoles of both the species
is saturable with a Km of 50 to 100 µM. Furthermore, the uptake of saponarin into vacuoles
from a barley mutant exhibiting a strongly reduced flavone glucoside
biosynthesis is drastically decreased when compared with the parent
variety. Thus, the barley vacuolar flavone glucoside/H+
antiporter could be modulated by the availability of the substrate. We
propose that different vacuolar transporters may be responsible for the
sequestration of species-specific/endogenous and nonspecific/xenobiotic secondary compounds in planta.
1
This work was supported by the Schweizer
Nationalfonds (grants to T.E., E.M., and M.K.), by the European Union
Biotech Program (grant no. BBW 97.0570 to N.F. and E.M.), and by the
Deutsche Forschungsgemeinschaft (to G.W.). M.K. was a Feodor-Lynen
Fellow supported by the Alexander-von-Humboldt Stiftung, Germany.
2
Present address: Recherche et Développement des
Plantes, Unité Mixte de Recherche 5667 Institut National de la
Recherche Agronomique-Centre National de la Recherche Scientifique-
Ecole Normal Supérieure de Lyon-Lyon 1, 46 Allée d'Italie,
F-69364 Lyon cedex 07, France.
*
Corresponding author; e-mail markus.klein{at}bota.unine.ch; fax
41-32-718-2271.
© 2002 American Society of Plant Physiologists
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