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Plant Physiol, March 2002, Vol. 128, pp. 1031-1045
Simultaneous Visualization of Peroxisomes and Cytoskeletal
Elements Reveals Actin and Not Microtubule-Based Peroxisome Motility in
Plants1,[w]
Jaideep
Mathur,
Neeta
Mathur, and
Martin
Hülskamp*
Botanical Institute III, University of Köln, Gyrhofstrase 15, 50931 Cologne, Germany
Peroxisomes were visualized in living plant cells using a yellow
fluorescent protein tagged with a peroxisomal targeting signal consisting of the SKL motif. Simultaneous visualization of peroxisomes and microfilaments/microtubules was accomplished in onion (Allium cepa) epidermal cells transiently expressing the yellow
fluorescent protein-peroxi construct, a green fluorescent
protein-mTalin construct that labels filamentous-actin filaments, and a
green fluorescent protein-microtubule-binding domain construct that
labels microtubules. The covisualization of peroxisomes and
cytoskeletal elements revealed that, contrary to the reports from
animal cells, peroxisomes in plants appear to associate with actin
filaments and not microtubules. That peroxisome movement is actin based
was shown by pharmacological studies. For this analysis we used onion
epidermal cells and various cell types of Arabidopsis including
trichomes, root hairs, and root cortex cells exhibiting different modes
of growth. In transient onion epidermis assay and in transgenic
Arabidopsis plants, an interference with the actin cytoskeleton
resulted in progressive loss of saltatory movement followed by the
aggregation and a complete cessation of peroxisome motility within 30 min of drug application. Microtubule depolymerization or stabilization
had no effect.
1
This work was supported by a Volkswagen Stiftung
grant (to M.H.).
[w]
The online version of this article contains Web-only
data. The supplemental material is available at
www.plantphysiol.org.
*
Corresponding author; e-mail martin.huelskamp{at}uni-koeln.de; fax
49-221-470-5062.
© 2002 American Society of Plant Physiologists
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