First published online February 24, 2002; 10.1104/pp.010744
Plant Physiol, March 2002, Vol. 128, pp. 1046-1056
Benzothiadiazole-Induced Priming for Potentiated Responses to
Pathogen Infection, Wounding, and Infiltration of Water into Leaves
Requires the NPR1/NIM1 Gene in Arabidopsis
Annegret
Kohler,1
Sandra
Schwindling,2 and
Uwe
Conrath*
Department of Biology, University of Kaiserslautern, D-67653
Kaiserslautern, Germany
Systemic acquired resistance (SAR) is a plant defense state that is
induced, for example, after previous pathogen infection or by chemicals
that mimic natural signaling compounds. SAR is associated with the
ability to induce cellular defense responses more rapidly and to a
greater degree than in noninduced plants, a process called
"priming." Arabidopsis plants were treated with the synthetic SAR
inducer benzothiadiazole (BTH) before stimulating two prominent
cellular defense responses, namely Phe AMMONIA-LYASE (PAL) gene activation and callose deposition. Although
BTH itself was essentially inactive at the immediate induction of these
two responses, the pretreatment with BTH greatly augmented the
subsequent PAL gene expression induced by
Pseudomonas syringae pv. tomato infection, wounding, or infiltrating the leaves with water. The BTH
pretreatment also enhanced the production of callose, which was induced
by wounding or infiltrating the leaves with water. It is interesting
that the potentiation by BTH pretreatment of PAL gene
activation and callose deposition was not seen in the Arabidopsis
nonexpresser of PR genes 1/noninducible immunity 1 mutant, which is compromised in SAR. In a converse manner, augmented PAL gene activation and enhanced callose biosynthesis
were found, without BTH pretreatment, in the Arabidopsis
constitutive expresser of
pathogenesis-related genes
(cpr)1 and constitutive expresser of pathogenesis-related genes 5 mutants, in
which SAR is constitutive. Moreover, priming for potentiated defense
gene activation was also found in pathogen-induced SAR. In sum, the
results suggest that priming is an important cellular mechanism in
acquired disease resistance of plants that requires the
nonexpresser of PR genes 1/noninducible immunity 1 gene.
1
Present address: Unité Mixte de Recherche
Institut National de la Recherche Agronomique-Université Henri
Poincaré Nancy, Interactions Arbres/Micro-Organismes, Institut
National de la Recherche Agronomique, Centre de Nancy, F-54280
Champenoux, France.
2
Present address: University of Saarland, Department of
Medical Biochemistry and Molecular Biology, Building 44, D-66421
Homburg, Germany.
*
Corresponding author; e-mail conrath{at}rhrk.uni-kl.de; fax
49-631-2052600.
© 2002 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
S. Van der Ent, B. W.M. Verhagen, R. Van Doorn, D. Bakker, M. G. Verlaan, M. J.C. Pel, R. G. Joosten, M. C.G. Proveniers, L.C. Van Loon, J. Ton, et al.
MYB72 Is Required in Early Signaling Steps of Rhizobacteria-Induced Systemic Resistance in Arabidopsis
Plant Physiology,
March 1, 2008;
146(3):
1293 - 1304.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Md. M. Hossain, F. Sultana, M. Kubota, H. Koyama, and M. Hyakumachi
The Plant Growth-Promoting Fungus Penicillium simplicissimum GP17-2 Induces Resistance in Arabidopsis thaliana by Activation of Multiple Defense Signals
Plant Cell Physiol.,
December 1, 2007;
48(12):
1724 - 1736.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Shimono, S. Sugano, A. Nakayama, C.-J. Jiang, K. Ono, S. Toki, and H. Takatsuji
Rice WRKY45 Plays a Crucial Role in Benzothiadiazole-Inducible Blast Resistance
PLANT CELL,
June 1, 2007;
19(6):
2064 - 2076.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I.-P. Ahn, S. Kim, Y.-H. Lee, and S.-C. Suh
Vitamin B1-Induced Priming Is Dependent on Hydrogen Peroxide and the NPR1 Gene in Arabidopsis
Plant Physiology,
February 1, 2007;
143(2):
838 - 848.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. van Hulten, M. Pelser, L. C. van Loon, C. M. J. Pieterse, and J. Ton
Costs and benefits of priming for defense in Arabidopsis
PNAS,
April 4, 2006;
103(14):
5602 - 5607.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I.-P. Ahn, S. Kim, and Y.-H. Lee
Vitamin B1 Functions as an Activator of Plant Disease Resistance
Plant Physiology,
July 1, 2005;
138(3):
1505 - 1515.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Ton, G. Jakab, V. Toquin, V. Flors, A. Iavicoli, M. N. Maeder, J.-P. Metraux, and B. Mauch-Mani
Dissecting the {beta}-Aminobutyric Acid-Induced Priming Phenomenon in Arabidopsis
PLANT CELL,
March 1, 2005;
17(3):
987 - 999.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Herms, K. Seehaus, H. Koehle, and U. Conrath
A Strobilurin Fungicide Enhances the Resistance of Tobacco against Tobacco Mosaic Virus and Pseudomonas syringae pv tabaci
Plant Physiology,
September 1, 2002;
130(1):
120 - 127.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Linke, U. Conrath, W. Jeblick, T. Betsche, A. Mahn, K. During, and H. E. Neuhaus
Inhibition of the Plastidic ATP/ADP Transporter Protein Primes Potato Tubers for Augmented Elicitation of Defense Responses and Enhances Their Resistance against Erwinia carotovora
Plant Physiology,
August 1, 2002;
129(4):
1607 - 1615.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
