Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online February 24, 2002; 10.1104/pp.010928

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
128/3/1057    most recent
pp.010928v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (55)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Qin, C.
Right arrow Articles by Wang, X.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Qin, C.
Right arrow Articles by Wang, X.
Agricola
Right arrow Articles by Qin, C.
Right arrow Articles by Wang, X.

Plant Physiol, March 2002, Vol. 128, pp. 1057-1068

The Arabidopsis Phospholipase D Family. Characterization of a Calcium-Independent and Phosphatidylcholine-Selective PLDzeta 1 with Distinct Regulatory Domains1

Chunbo Qin and Xuemin Wang*

Department of Biochemistry, Kansas State University, Manhattan, Kansas 66506

Four types of phospholipase D (PLD), PLDalpha , beta , gamma , and delta , have been characterized in Arabidopsis, and they display different requirements for Ca2+, phosphatidylinositol 4,5-bisphosphate (PIP2), substrate vesicle composition, and/or free fatty acids. However, all previously cloned plant PLDs contain a Ca2+-dependent phospholipid-binding C2 domain and require Ca2+ for activity. This study documents a new type of PLD, PLDzeta 1, which is distinctively different from previously characterized PLDs. It contains at the N terminus a Phox homology domain and a pleckstrin homology domain, but not the C2 domain. A full-length cDNA for Arabidopsis PLDzeta 1 has been identified and used to express catalytically active PLD in Escherichia coli. PLDzeta 1 does not require Ca2+ or any other divalent cation for activity. In addition, it selectively hydrolyzes phosphatidylcholine, whereas the other Arabidopsis PLDs use several phospholipids as substrates. PLDzeta 1 requires PIP2 for activity, but unlike the PIP2-requiring PLDbeta or gamma , phosphatidylethanolamine is not needed in substrate vesicles. These differences are described, together with a genomic analysis of 12 putative Arabidopsis PLD genes that are grouped into alpha , beta , delta , gamma , and zeta  based on their gene architectures, sequence similarities, domain structures, and biochemical properties.

1 This work was supported by the National Science Foundation (grant no. IBN-9808729) and the U.S. Department of Agriculture (2001-35304-10087). This is contribution 02-142-J of the Kansas Agricultural Experiment Station.

* Corresponding author; e-mail wangs{at}ksu.edu; fax 785-532-7278.

© 2002 American Society of Plant Physiologists


This article has been cited by other articles:


Home page
 Mol PlantHome page
Y. Hong, S. Zheng, and X. Wang
Dual Functions of Phospholipase D{alpha}1 in Plant Response to Drought
Mol Plant, March 1, 2008; 1(2): 262 - 269.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. Hong, X. Pan, R. Welti, and X. Wang
Phospholipase D{alpha}3 Is Involved in the Hyperosmotic Response in Arabidopsis
PLANT CELL, March 1, 2008; 20(3): 803 - 816.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. Kusano, C. Testerink, J. E.M. Vermeer, T. Tsuge, H. Shimada, A. Oka, T. Munnik, and T. Aoyama
The Arabidopsis Phosphatidylinositol Phosphate 5-Kinase PIP5K3 Is a Key Regulator of Root Hair Tip Growth
PLANT CELL, February 1, 2008; 20(2): 367 - 380.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Li, R. Wang, M. Li, L. Li, C. Wang, R. Welti, and X. Wang
Differential Degradation of Extraplastidic and Plastidic Lipids during Freezing and Post-freezing Recovery in Arabidopsis thaliana
J. Biol. Chem., January 4, 2008; 283(1): 461 - 468.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
P.-L. Jiang, C.-S. Wang, C.-M. Hsu, G.-Y. Jauh, and J. T. C. Tzen
Stable Oil Bodies Sheltered by a Unique Oleosin in Lily Pollen
Plant Cell Physiol., June 1, 2007; 48(6): 812 - 821.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. J. Benschop, F. F. Millenaar, M. E. Smeets, M. van Zanten, L. A.C.J. Voesenek, and A. J.M. Peeters
Abscisic Acid Antagonizes Ethylene-Induced Hyponastic Growth in Arabidopsis
Plant Physiology, February 1, 2007; 143(2): 1013 - 1023.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
G. Li and H.-W. Xue
Arabidopsis PLD{zeta}2 Regulates Vesicle Trafficking and Is Required for Auxin Response
PLANT CELL, January 1, 2007; 19(1): 281 - 295.
[Abstract] [Full Text] [PDF]

Home page
 Protein Eng Des SelHome page
A. Lerchner, J. Mansfeld, K. Kuppe, and R. Ulbrich-Hofmann
Probing conserved amino acids in phospholipase D (Brassica oleracea var. capitata) for their importance in hydrolysis and transphosphatidylation activity
Protein Eng. Des. Sel., October 1, 2006; 19(10): 443 - 452.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Li, R. Welti, and X. Wang
Quantitative Profiling of Arabidopsis Polar Glycerolipids in Response to Phosphorus Starvation. Roles of Phospholipases D{zeta}1 and D{zeta}2 in Phosphatidylcholine Hydrolysis and Digalactosyldiacylglycerol Accumulation in Phosphorus-Starved Plants
Plant Physiology, October 1, 2006; 142(2): 750 - 761.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
R. M.P. Siloto, K. Findlay, A. Lopez-Villalobos, E. C. Yeung, C. L. Nykiforuk, and M. M. Moloney
The Accumulation of Oleosins Determines the Size of Seed Oilbodies in Arabidopsis
PLANT CELL, August 1, 2006; 18(8): 1961 - 1974.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Cruz-Ramirez, A. Oropeza-Aburto, F. Razo-Hernandez, E. Ramirez-Chavez, and L. Herrera-Estrella
Phospholipase DZ2 plays an important role in extraplastidic galactolipid biosynthesis and phosphate recycling in Arabidopsis roots
PNAS, April 25, 2006; 103(17): 6765 - 6770.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. A. Braybrook, S. L. Stone, S. Park, A. Q. Bui, B. H. Le, R. L. Fischer, R. B. Goldberg, and J. J. Harada
Genes directly regulated by LEAFY COTYLEDON2 provide insight into the control of embryo maturation and somatic embryogenesis
PNAS, February 28, 2006; 103(9): 3468 - 3473.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Li, C. Qin, R. Welti, and X. Wang
Double Knockouts of Phospholipases D{zeta}1 and D{zeta}2 in Arabidopsis Affect Root Elongation during Phosphate-Limited Growth But Do Not Affect Root Hair Patterning
Plant Physiology, February 1, 2006; 140(2): 761 - 770.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Vergnolle, M.-N. Vaultier, L. Taconnat, J.-P. Renou, J.-C. Kader, A. Zachowski, and E. Ruelland
The Cold-Induced Early Activation of Phospholipase C and D Pathways Determines the Response of Two Distinct Clusters of Genes in Arabidopsis Cell Suspensions
Plant Physiology, November 1, 2005; 139(3): 1217 - 1233.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
X. Wang
Regulatory Functions of Phospholipase D and Phosphatidic Acid in Plant Growth, Development, and Stress Responses
Plant Physiology, October 1, 2005; 139(2): 566 - 573.
[Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. Waltermann and A. Steinbuchel
Neutral Lipid Bodies in Prokaryotes: Recent Insights into Structure, Formation, and Relationship to Eukaryotic Lipid Depots
J. Bacteriol., June 1, 2005; 187(11): 3607 - 3619.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Horan, J. Lauricha, J. Bailey-Serres, N. Raikhel, and T. Girke
Genome Cluster Database. A Sequence Family Analysis Platform for Arabidopsis and Rice
Plant Physiology, May 1, 2005; 138(1): 47 - 54.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Nakamura, K. Awai, T. Masuda, Y. Yoshioka, K.-i. Takamiya, and H. Ohta
A Novel Phosphatidylcholine-hydrolyzing Phospholipase C Induced by Phosphate Starvation in Arabidopsis
J. Biol. Chem., March 4, 2005; 280(9): 7469 - 7476.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Pappan, L. Zheng, R. Krishnamoorthi, and X. Wang
Evidence for and Characterization of Ca2+ Binding to the Catalytic Region of Arabidopsis thaliana Phospholipase D{beta}
J. Biol. Chem., November 12, 2004; 279(46): 47833 - 47839.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Charron, J.-L. Pingret, M. Chabaud, E.-P. Journet, and D. G. Barker
Pharmacological Evidence That Multiple Phospholipid Signaling Pathways Link Rhizobium Nodulation Factor Perception in Medicago truncatula Root Hairs to Intracellular Responses, Including Ca2+ Spiking and Specific ENOD Gene Expression
Plant Physiology, November 1, 2004; 136(3): 3582 - 3593.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Yamaguchi, S. Tanabe, E. Minami, and N. Shibuya
Activation of Phospholipase D Induced by Hydrogen Peroxide in Suspension-cultured Rice Cells
Plant Cell Physiol., September 15, 2004; 45(9): 1261 - 1270.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. Cruz-Ramirez, J. Lopez-Bucio, G. Ramirez-Pimentel, A. Zurita-Silva, L. Sanchez-Calderon, E. Ramirez-Chavez, E. Gonzalez-Ortega, and L. Herrera-Estrella
The xipotl Mutant of Arabidopsis Reveals a Critical Role for Phospholipid Metabolism in Root System Development and Epidermal Cell Integrity
PLANT CELL, August 1, 2004; 16(8): 2020 - 2034.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
W. Zhang, C. Qin, J. Zhao, and X. Wang
Phospholipase D{alpha}1-derived phosphatidic acid interacts with ABI1 phosphatase 2C and regulates abscisic acid signaling
PNAS, June 22, 2004; 101(25): 9508 - 9513.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Thiery, A.-S. Leprince, D. Lefebvre, M. A. Ghars, E. Debarbieux, and A. Savoure
Phospholipase D Is a Negative Regulator of Proline Biosynthesis in Arabidopsis thaliana
J. Biol. Chem., April 9, 2004; 279(15): 14812 - 14818.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Zhao and X. Wang
Arabidopsis Phospholipase D{alpha}1 Interacts with the Heterotrimeric G-protein {alpha}-Subunit through a Motif Analogous to the DRY Motif in G-protein-coupled Receptors
J. Biol. Chem., January 16, 2004; 279(3): 1794 - 1800.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Zambonelli, M. Casali, and M. F. Roberts
Mutagenesis of Putative Catalytic and Regulatory Residues of Streptomyces chromofuscus Phospholipase D Differentially Modifies Phosphatase and Phosphodiesterase Activities
J. Biol. Chem., December 26, 2003; 278(52): 52282 - 52289.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
P. Dhonukshe, A. M. Laxalt, J. Goedhart, T. W. J. Gadella, and T. Munnik
Phospholipase D Activation Correlates with Microtubule Reorganization in Living Plant Cells
PLANT CELL, November 1, 2003; 15(11): 2666 - 2679.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
J. D. McGee, J. L. Roe, T. A. Sweat, X. Wang, J. A. Guikema, and J. E. Leach
Rice Phospholipase D Isoforms Show Differential Cellular Location and Gene Induction
Plant Cell Physiol., October 15, 2003; 44(10): 1013 - 1026.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
W. Zhang, C. Wang, C. Qin, T. Wood, G. Olafsdottir, R. Welti, and X. Wang
The Oleate-Stimulated Phospholipase D, PLD{delta}, and Phosphatidic Acid Decrease H2O2-Induced Cell Death in Arabidopsis
PLANT CELL, October 1, 2003; 15(10): 2285 - 2295.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
J. Gardiner, D. A. Collings, J. D. I. Harper, and J. Marc
The Effects of the Phospholipase D-Antagonist 1-Butanol on Seedling Development and Microtubule Organisation in Arabidopsis
Plant Cell Physiol., July 15, 2003; 44(7): 687 - 696.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
Y. Ohashi, A. Oka, R. Rodrigues-Pousada, M. Possenti, I. Ruberti, G. Morelli, and T. Aoyama
Modulation of Phospholipid Signaling by GLABRA2 in Root-Hair Pattern Formation
Science, May 30, 2003; 300(5624): 1427 - 1430.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. den Hartog, N. Verhoef, and T. Munnik
Nod Factor and Elicitors Activate Different Phospholipid Signaling Pathways in Suspension-Cultured Alfalfa Cells
Plant Physiology, May 1, 2003; 132(1): 311 - 317.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
R. C. W. Hou, M. Y. Lin, M. M. C. Wang, and J. T. C. Tzen
Increase of Viability of Entrapped Cells of Lactobacillus delbrueckii ssp. bulgaricus in Artificial Sesame Oil Emulsions
J Dairy Sci, February 1, 2003; 86(2): 424 - 428.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Qin, C. Wang, and X. Wang
Kinetic Analysis of Arabidopsis Phospholipase Ddelta . SUBSTRATE PREFERENCE AND MECHANISM OF ACTIVATION BY Ca2+ AND PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE
J. Biol. Chem., December 13, 2002; 277(51): 49685 - 49690.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Welti, W. Li, M. Li, Y. Sang, H. Biesiada, H.-E Zhou, C. B. Rajashekar, T. D. Williams, and X. Wang
Profiling Membrane Lipids in Plant Stress Responses. ROLE OF PHOSPHOLIPASE Dalpha IN FREEZING-INDUCED LIPID CHANGES IN ARABIDOPSIS
J. Biol. Chem., August 23, 2002; 277(35): 31994 - 32002.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2002 by the American Society of Plant Biologists