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First published online February 24, 2002; 10.1104/pp.010887

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Plant Physiol, March 2002, Vol. 128, pp. 1109-1119

gamma -Glutamyl Transpeptidase in Transgenic Tobacco Plants. Cellular Localization, Processing, and Biochemical Properties1

Sergei Storozhenko, Enric Belles-Boix,2 Elena Babiychuk, Didier Hérouart, Mark W. Davey,3 Luit Slooten, Marc Van Montagu, Dirk Inzé,* and Sergei Kushnir

Vakgroep Moleculaire Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium (S.S., E.B.-B., E.B., M.W.D., M.V.M., D.I., S.K.); Laboratoire de Biologie Végétale et Microbiologie, Université de Nice, F-06108 Nice cedex 2, France (D.H.); and Laboratorium voor Biofysica, Vrije Universiteit Brussel, B-1050 Brussels, Belgium (L.S.)

gamma -Glutamyl transpeptidase (gamma -GT) is a ubiquitous enzyme that catalyzes the first step of glutathione (GSH) degradation in the gamma -glutamyl cycle in mammals. A cDNA encoding an Arabidopsis homolog for gamma -GT was overexpressed in tobacco (Nicotiana tabacum) plants. A high level of the membrane-bound gamma -GT activity was localized outside the cell in transgenic plants. The overproduced enzyme was characterized by a high affinity to GSH and was cleaved post-translationally in two unequal subunits. Thus, Arabidopsis gamma -GT is similar to the mammalian enzymes in enzymatic properties, post-translational processing, and cellular localization, suggesting analogous biological functions as a key enzyme in the catabolism of GSH.

1 This work was supported by the Fund for Scientific Research Flanders (grant no. G004796).

2 Present address: Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, Route de St-Cyr, F-78026 Versailles cedex, France.

3 Present address: Fruitteeltcentrum, Willem de Croylaan 442, B-3001 Heverlee, Belgium.

* Corresponding author; e-mail diinz{at}gengenp.rug.ac.be; fax 32-9-264-5349.

© 2002 American Society of Plant Physiologists


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