First published online February 24, 2002; 10.1104/pp.010646
Plant Physiol, March 2002, Vol. 128, pp. 865-875
Copper Amine Oxidase Expression in Defense Responses to
Wounding and Ascochyta rabiei Invasion1
Giuseppina
Rea,
Ouissal
Metoui,
Alessandro
Infantino,
Rodolfo
Federico, and
Riccardo
Angelini*
Dipartimento di Biologia, Università degli Studi
"Roma Tre," Viale Guglielmo Marconi 446, 00146 Rome, Italy; and
Istituto Sperimentale per la Patologia Vegetale, Via Carlo
Giuseppe Bertero 22, 00156 Rome, Italy
Wounding chickpea (Cicer arietinum)
internodes or cotyledons resulted in an increase in the steady-state
level of copper amine oxidase (CuAO) expression both locally and
systemically. Dissection of the molecular mechanisms controlling CuAO
expression indicated that jasmonic acid worked as a potent inducer of
the basal and wound-inducible CuAO expression, whereas salicylic acid
and abscisic acid caused a strong reduction of the wound-induced CuAO
expression, without having any effect on the basal levels. Epicotyl
treatment with the CuAO mechanism-based inhibitor 2-bromoethylamine
decreased hydrogen peroxide (H2O2) levels in
all the internodes, as evidenced in vivo by 3,3'-diaminobenzidine
oxidation. Moreover, inhibitor pretreatment of wounded epicotyls
resulted in a lower accumulation of H2O2 both
at the wound site and in distal organs. In vivo CuAO inhibition by
2-bromoethylamine after inoculation of resistant chickpea cv Sultano
with Ascochyta rabiei resulted in the development of
extended necrotic lesions, with extensive cell damage occurring in
sclerenchyma and cortical parenchyma tissues. These results, besides
stressing the fine-tuning by key signaling molecules in wound-induced
CuAO regulation, demonstrate that local and systemic CuAO induction is
essential for H2O2 production in response to wounding and indicate the relevance of these enzymes in protection against pathogens.
1
This work was supported by the Italian Ministry
for University and Scientific Research and by the European Commission
(project Faba Bean Resistance and Yield in Mediterranean Area,
contract no. 18-CT 98-0300, as part of O.M.'s doctorate thesis).
*
Corresponding author; e-mail angelini{at}bio.uniroma3.it; fax 0655176321.
© 2002 American Society of Plant Physiologists
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