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Plant Physiol, March 2002, Vol. 128, pp. 876-884
Evidence Supporting a Role of Jasmonic Acid in Arabidopsis Leaf
Senescence1
Yuehui
He,
Hirotada
Fukushige,
David F.
Hildebrand, and
Susheng
Gan*
Plant Physiology/Biochemistry/Molecular Biology Program, Department
of Agronomy, Agricultural Sciences Center-North, University of
Kentucky, Lexington, Kentucky 40546-0091 (Y.H., H.F., D.F.H., S.G.);
and the Tobacco and Health Research Institute, University of Kentucky,
Lexington, Kentucky 40546-0236 (Y.H., S.G.)
In this work, the role of jasmonic acid (JA) in leaf
senescence is examined. Exogenous application of JA caused premature senescence in attached and detached leaves in wild-type Arabidopsis but
failed to induce precocious senescence of JA-insensitive mutant coi1 plants, suggesting that the JA-signaling pathway is
required for JA to promote leaf senescence. JA levels in senescing
leaves are 4-fold higher than in non-senescing ones. Concurrent with the increase in JA level in senescing leaves, genes encoding the enzymes that catalyze most of the reactions of the JA biosynthetic pathway are differentially activated during leaf senescence in Arabidopsis, except for allene oxide synthase, which is
constitutively and highly expressed throughout leaf development.
Arabidopsis lipoxygenase 1 (cytoplasmic) expression is
greatly increased but lipoxygenase 2 (plastidial)
expression is sharply reduced during leaf senescence. Similarly,
AOC1 (allene oxide cyclase 1),
AOC2, and AOC3 are all up-regulated,
whereas AOC4 is down-regulated with the progression of
leaf senescence. The transcript levels of 12-oxo-PDA reductase
1 and 12-oxo-PDA reductase 3 also increase in
senescing leaves, as does PED1 (encoding a
3-keto-acyl-thiolase for  -oxidation). This represents the first
report, to our knowledge, of an increase in JA levels and expression of
oxylipin genes during leaf senescence, and indicates that JA may play a
role in the senescence program.
1
This work was supported by the U.S. Department
of Agriculture-National Research Initiative Competitive Grants Program
(grant nos. 2001-35304-09994 to S.G. and 9701487 to D.F.H.) and by
the Tobacco and Health Research Institute's Biotechnology Program at
the University of Kentucky (grants to S.G. and D.F.H.). Y.H. was
supported in part by the University of Kentucky Research Challenge Trust Fund (Plant Sciences).
*
Corresponding author; e-mail sgan{at}uky.edu; fax
859-323-1077.
© 2002 American Society of Plant Physiologists
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