First published online February 24, 2002; 10.1104/pp.010685
Plant Physiol, March 2002, Vol. 128, pp. 951-961
Snakin-2, an Antimicrobial Peptide from Potato Whose Gene Is
Locally Induced by Wounding and Responds to Pathogen
Infection1
Marta
Berrocal-Lobo,
Ana
Segura,2
Manuel
Moreno,
Gemma
López,
Francisco
García-Olmedo, and
Antonio
Molina*
Laboratorio de Bioquímica y Biología Molecular,
Departamento de Biotecnología-Universidad Politecnica
Madrid, Escuela Tecnica Superior Ingenieros Agrónomos, E-28040
Madrid, Spain
The peptide snakin-2 (StSN2) has been isolated from potato
(Solanum tuberosum cv Jaerla) tubers and found to be
active (EC50 = 1-20 µM) against
fungal and bacterial plant pathogens. It causes a rapid aggregation of
both Gram-positive and Gram-negative bacteria. The corresponding
StSN2 cDNA encodes a signal sequence followed by a
15-residue acidic sequence that precedes the mature StSN2 peptide,
which is basic (isoelectric point = 9.16) and 66 amino acid
residues long (molecular weight of 7,025). The StSN2
gene is developmentally expressed in tubers, stems, flowers, shoot apex, and leaves, but not in roots, or stolons, and is locally up-regulated by wounding and by abscisic acid treatment. Expression of
this gene is also up-regulated after infection of potato tubers with
the compatible fungus Botritys cinerea and
down-regulated by the virulent bacteria Ralstonia
solanacearum and Erwinia chrysanthemi. These
observations are congruent with the hypothesis that the StSN2 is a
component of both constitutive and inducible defense barriers.
1
This work was supported by the Dirección
General de Investigacion Cientifica y Technica (grant no. PB92-0325 to
F.G.-O.), by the Comunidad de Madrid (grant no. 07B/0002/1999 to A.M.), by the Ministerio de Ciencia y Tecnología from Spain (grant no. BIO2000-1308 to A.M), and by the European Project BIOCT97-2120 (DGXII-SSMI to M.B.-L.).
2
Present address: Estación Experimental del
Zaidín, Consejo Superior de Investigaciones
Científicas, Granada, Spain.
*
Corresponding author; e-mail molina{at}bit.etsia.upm.es; fax
34-91-3365757.
© 2002 American Society of Plant Physiologists
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