First published online March 7, 2002; 10.1104/pp.010925
Plant Physiol, April 2002, Vol. 128, pp. 1180-1188
Guard Cell- and Phloem Idioblast-Specific Expression of
Thioglucoside Glucohydrolase 1 (Myrosinase) in
Arabidopsis1
Harald
Husebye,2
Supachitra
Chadchawan,2
Per
Winge,
Ole P.
Thangstad, and
Atle M.
Bones*
Department of Botany, Norwegian University of Science and
Technology, N-7491 Trondheim, Norway (H.H., P.W., O.P.T., A.M.B.); and
Department of Botany, Chulalongkorn University, Bangkok 10330, Thailand (S.C.)
Thioglucoside glucohydrolase 1 (TGG1) is one of two known
functional myrosinase enzymes in Arabidopsis. The enzyme catalyzes the
hydrolysis of glucosinolates into compounds that are toxic to various
microbes and herbivores. Transgenic Arabidopsis plants carrying
 -glucuronidase and green fluorescent protein reporter genes fused to
0.5 or 2.5 kb of the TGG1 promoter region were used to study spatial
promoter activity. Promoter activity was found to be highly specific
and restricted to guard cells and distinct cells of the phloem. No
promoter activity was detected in the root or seed. All guard cells
show promoter activity. Positive phloem cells are distributed in a
discontinuous pattern and occur more frequent in young tissues.
Immunocytochemical localization of myrosinase in transverse and
longitudinal sections of embedded material show that the TGG1
promoter activity reflects the position of the myrosinase enzyme. In
the flower stalk, the myrosinase-containing phloem cells are located
between phloem sieve elements and glucosinolate-rich S cells. Our
results suggest a cellular separation of myrosinase enzyme and
glucosinolate substrate, and that myrosinase is contained in distinct
cells. We discuss the potential advantages of locating defense and
communication systems to only a few specific cell types.
1
This work was supported by Nordisk Kontaktorgan
for Jorbruksforkning (grant no. 104).
2
These authors contributed equally to this work.
*
Corresponding author; e-mail atle.bones{at}chembio.ntnu.no; fax
47-73-596100.
© 2002 American Society of Plant Physiologists
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