First published online April 9, 2002; 10.1104/pp.010678
Plant Physiol, April 2002, Vol. 128, pp. 1223-1233
Functional Properties and Regulatory Complexity of a Minimal
RBCS Light-Responsive Unit Activated by Phytochrome,
Cryptochrome, and Plastid Signals1
Aída
Martínez-Hernández,
Luisa
López-Ochoa,
Gerardo
Argüello-Astorga,2 and
Luis
Herrera-Estrella*
Departamento de Ingeniería Genética de Plantas,
Centro de Investigación y Estudios Avanzados del Instituto
Politécnico Nacional, Apartado Postal 629, 36500 Irapuato,
Guanajuato, México
Light-inducible promoters are able to respond to a wide
spectrum of light through multiple photoreceptor systems. Several cis-acting elements have been identified as components of
light-responsive promoter elements; however, none of these regulatory
elements by itself appears to be sufficient to confer light
responsiveness; rather, the combination of at least two elements seems
to be required. Using phylogenetic structural analysis, we have
identified conserved DNA modular arrays (CMAs) associated with
light-responsive promoter regions that have been conserved throughout
the evolutionary radiation of angiosperms. Here, we report the
functional characterization of CMA5, a native 52-bp fragment of the
Nicotiana plumbaginifolia rbcS 8B promoter, which
contains an I- and a G-box cis-element. CMA5 behaves as a
light-responsive minimal unit capable of activating a heterologous
minimal promoter in a phytochrome-, cryptochrome-, and
plastid-dependent manner. We also show that CMA5 light induction requires HY5 and that downstream negative regulators COP (constitutive photomorphogenic)/DET (de-etiolated) regulate its activity. Our results
show that the simplest light-responsive promoter element from
photosynthesis-associated genes described to date is the common target
for different signals involved in light regulation. The possible
mechanism involved in light-transcriptional regulation and tissue
specificity of combinatorial elements units is discussed.
1
This work was supported in part by the Howard
Hughes Medical Institute (grant no. 75191-526901 to L.H.-E.) and by
Consejo Nacional de Ciencia y Tecnología-México
(doctoral fellowship to A.M.-H.).
2
Present address: Departamento de Biologia Molecular,
Instituto Potosino de Investigaciones Científicas y
Tecnológicas, Avenida Venustiano Carranza 2425, 78270 San
Luis Potosí, San Luis Potosí, Mexico.
*
Corresponding author; e-mail lherrera{at}ira.cinvestav.mx; fax
52-462-6245849.
© 2002 American Society of Plant Physiologists
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