First published online February 28, 2002; 10.1104/pp.010731
Plant Physiol, April 2002, Vol. 128, pp. 1359-1367
Influence of Iron Status on Cadmium and Zinc Uptake by
Different Ecotypes of the Hyperaccumulator Thlaspi
caerulescens1
Enzo
Lombi,
Kathryn L.
Tearall,
Jonathan R.
Howarth,
Fang-Jie
Zhao,
Malcolm J.
Hawkesford, and
Steve P.
McGrath*
Agriculture and Environment Division, IACR-Rothamsted, Harpenden,
Hertsfordshire AL5 2JQ, United Kingdom
We have previously identified an ecotype of the
hyperaccumulator Thlaspi caerulescens (Ganges), which is
far superior to other ecotypes (including Prayon) in Cd uptake. In this
study, we investigated the effect of Fe status on the uptake of Cd and
Zn in the Ganges and Prayon ecotypes, and the kinetics of Cd and Zn
influx using radioisotopes. Furthermore, the T.
caerulescens ZIP (Zn-regulated transporter/Fe-regulated
transporter-like protein) genes TcZNT1-G and
TcIRT1-G were cloned from the Ganges ecotype and their
expression under Fe-sufficient and -deficient conditions was analyzed.
Both short- and long-term studies revealed that Cd uptake was
significantly enhanced by Fe deficiency only in the Ganges ecotype. The
concentration-dependent kinetics of Cd influx showed that the
Vmax of Cd was 3 times greater in
Fe-deficient Ganges plants compared with Fe-sufficient plants. In
Prayon, Fe deficiency did not induce a significant increase in
Vmax for Cd. Zn uptake was not influenced by
the Fe status of the plants in either of the ecotypes. These results
are in agreement with the gene expression study. The abundance of
ZNT1-G mRNA was similar between the Fe treatments and
between the two ecotypes. In contrast, abundance of the
TcIRT1-G mRNA was greatly increased only in Ganges root
tissue under Fe-deficient conditions. The present results indicate that
the stimulatory effect of Fe deficiency on Cd uptake in Ganges may be
related to an up-regulation in the expression of genes encoding for
Fe2+ uptake, possibly TcIRT1-G.
1
This work was supported by the
Directorate General XII of the European Commission, by Rio Tinto
Technology, and by the Biotechnology and Biological Sciences Research
Council of the UK (grant to IACR-Rothamsted).
*
Corresponding author; e-mail steve.mcgrath{at}bbsrc.ac.uk; fax
44-1582-760981.
© 2002 American Society of Plant Physiologists
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