First published online March 22, 2002; 10.1104/pp.010918
Plant Physiol, May 2002, Vol. 129, pp. 191-200
Gibberellin Signaling in Barley Aleurone Cells. Control of SLN1
and GAMYB Expression
Frank
Gubler,*
Peter Michael
Chandler,
Rosemary G.
White,
Danny J.
Llewellyn, and
John V.
Jacobsen
Commonwealth Scientific and Industrial Research
Organization, Plant Industry, G.P.O. Box 1600, Canberra,
Australian Capital Territory 2601, Australia
We have previously identified GAMYB, a gibberellin
(GA)-regulated transcriptional activator of  -amylase gene
expression, in aleurone cells of barley (Hordeum
vulgare). To examine the regulation of GAMYB expression, we
describe the use of nuclear run-on experiments to show that GA causes a
2-fold increase in the rate of GAMYB transcription and that the effect
of GA can be blocked by abscisic acid (ABA). To identify
GA-signaling components that regulate GAMYB expression, we examined the
role of SLN1, a negative regulator of GA signaling in barley. SLN1,
which is the product of the Sln1
(Slender1) locus, is necessary for repression of GAMYB
in barley aleurone cells. The activity of SLN1 in aleurone cells is
regulated posttranslationally. SLN1 protein levels decline rapidly in
response to GA before any increase in GAMYB levels. Green fluorescent
protein-SLN1 fusion protein was targeted to the nucleus of
aleurone protoplasts and disappeared in response to GA. Evidence from a
dominant dwarf mutant at Sln1, and from the
gse1 mutant (that affects GA "sensitivity"),
indicates that GA acts by regulating SLN1 degradation and not
translation. Mutation of the DELLA region of SLN1 results in
increased protein stability in GA-treated layers, indicating that the
DELLA region plays an important role in GA-induced degradation
of SLN1. Unlike GA, ABA had no effect on SLN1 stability, confirming
that ABA acts downstream of SLN1 to block GA signaling.
*
Corresponding author; e-mail
frank.gubler{at}csiro.au; fax 61-0262465000.
© 2002 American Society of Plant Physiologists
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