Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (44)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shabala, S. N.
Right arrow Articles by Lew, R. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shabala, S. N.
Right arrow Articles by Lew, R. R.
Agricola
Right arrow Articles by Shabala, S. N.
Right arrow Articles by Lew, R. R.

Plant Physiol, May 2002, Vol. 129, pp. 290-299

Turgor Regulation in Osmotically Stressed Arabidopsis Epidermal Root Cells. Direct Support for the Role of Inorganic Ion Uptake as Revealed by Concurrent Flux and Cell Turgor Measurements1

Sergey N. Shabala and Roger R. Lew*

School of Agricultural Science, University of Tasmania, Hobart, Australia (S.N.S.); and Department of Biology, York University, Toronto, Canada M3J 1P3 (R.R.L.)

Hyperosmotic stress is known to significantly enhance net uptake of inorganic ions into plant cells. Direct evidence for cell turgor recovery via such a mechanism, however, is still lacking. In the present study, we performed concurrent measurements of net ion fluxes (with the noninvasive microelectrode ion flux estimation technique) and cell turgor changes (with the pressure-probe technique) to provide direct evidence that inorganic ion uptake regulates turgor in osmotically stressed Arabidopsis epidermal root cells. Immediately after onset of hyperosmotic stress (100/100 mM mannitol/sorbitol treatment), the cell turgor dropped from 0.65 to about 0.25 MPa. Turgor recovery started within 2 to 10 min after the treatment and was accompanied by a significant (30-80 nmol m-2 s-1) increase in uptake of K+, Cl-, and Na+ by root cells. In most cells, almost complete (>90% of initial values) recovery of the cell turgor was observed within 40 to 50 min after stress onset. In another set of experiments, we combined the voltage-clamp and the microelectrode ion flux estimation techniques to show that this process is, in part, mediated by voltage-gated K+ transporters at the cell plasma membrane. The possible physiological significance of these findings is discussed.

1 This work was supported by the Australian Research Council (grant to S.N.S.) and by the Canadian Natural Sciences and Engineering Research Council (grant to R.R.L.).

* Corresponding author; e-mail planters{at}yorku.ca; fax 416-736-5698.

© 2002 American Society of Plant Physiologists


This article has been cited by other articles:


Home page
 J Exp BotHome page
S. Shabala
Salinity and programmed cell death: unravelling mechanisms for ion specific signalling
J. Exp. Bot., March 1, 2009; 60(3): 709 - 712.
[Full Text] [PDF]

Home page
 J Exp BotHome page
T. A. Cuin, S. A. Betts, R. Chalmandrier, and S. Shabala
A root's ability to retain K+ correlates with salt tolerance in wheat
J. Exp. Bot., July 1, 2008; 59(10): 2697 - 2706.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Z. Chen, T. A. Cuin, M. Zhou, A. Twomey, B. P. Naidu, and S. Shabala
Compatible solute accumulation and stress-mitigating effects in barley genotypes contrasting in their salt tolerance
J. Exp. Bot., December 1, 2007; 58(15-16): 4245 - 4255.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Z. Chen, I. I. Pottosin, T. A. Cuin, A. T. Fuglsang, M. Tester, D. Jha, I. Zepeda-Jazo, M. Zhou, M. G. Palmgren, I. A. Newman, et al.
Root Plasma Membrane Transporters Controlling K+/Na+ Homeostasis in Salt-Stressed Barley
Plant Physiology, December 1, 2007; 145(4): 1714 - 1725.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
R. R. Lew
Ionic currents and ion fluxes in Neurospora crassa hyphae
J. Exp. Bot., September 26, 2007; (2007) erm204v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Pang, T. Cuin, L. Shabala, M. Zhou, N. Mendham, and S. Shabala
Effect of Secondary Metabolites Associated with Anaerobic Soil Conditions on Ion Fluxes and Electrophysiology in Barley Roots
Plant Physiology, September 1, 2007; 145(1): 266 - 276.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Gagneul, A. Ainouche, C. Duhaze, R. Lugan, F. R. Larher, and A. Bouchereau
A Reassessment of the Function of the So-Called Compatible Solutes in the Halophytic Plumbaginaceae Limonium latifolium
Plant Physiology, July 1, 2007; 144(3): 1598 - 1611.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
R. R. Lew and N. N. Levina
Turgor regulation in the osmosensitive cut mutant of Neurospora crassa
Microbiology, May 1, 2007; 153(5): 1530 - 1537.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. T. Fuglsang, Y. Guo, T. A. Cuin, Q. Qiu, C. Song, K. A. Kristiansen, K. Bych, A. Schulz, S. Shabala, K. S. Schumaker, et al.
Arabidopsis Protein Kinase PKS5 Inhibits the Plasma Membrane H+-ATPase by Preventing Interaction with 14-3-3 Protein
PLANT CELL, May 1, 2007; 19(5): 1617 - 1634.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Shabala, V. Demidchik, L. Shabala, T. A. Cuin, S. J. Smith, A. J. Miller, J. M. Davies, and I. A. Newman
Extracellular Ca2+ Ameliorates NaCl-Induced K+ Loss from Arabidopsis Root and Leaf Cells by Controlling Plasma Membrane K+-Permeable Channels
Plant Physiology, August 1, 2006; 141(4): 1653 - 1665.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
R. R. Lew, N. N. Levina, L. Shabala, M. I. Anderca, and S. N. Shabala
Role of a Mitogen-Activated Protein Kinase Cascade in Ion Flux-Mediated Turgor Regulation in Fungi
Eukaryot. Cell, March 1, 2006; 5(3): 480 - 487.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Shabala, L. Shabala, D. Gradmann, Z. Chen, I. Newman, and S. Mancuso
Oscillations in plant membrane transport: model predictions, experimental validation, and physiological implications
J. Exp. Bot., January 1, 2006; 57(1): 171 - 184.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. A. Cuin and S. Shabala
Exogenously Supplied Compatible Solutes Rapidly Ameliorate NaCl-induced Potassium Efflux from Barley Roots
Plant Cell Physiol., December 1, 2005; 46(12): 1924 - 1933.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
R. R. Lew
Mass flow and pressure-driven hyphal extension in Neurospora crassa
Microbiology, August 1, 2005; 151(8): 2685 - 2692.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
R. S. Tegg, L. Melian, C. R. Wilson, and S. Shabala
Plant Cell Growth and Ion Flux Responses to the Streptomycete Phytotoxin Thaxtomin A: Calcium and Hydrogen Flux Patterns Revealed by the Non-invasive MIFE Technique
Plant Cell Physiol., April 1, 2005; 46(4): 638 - 648.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Z. Qi, A. Kishigami, Y. Nakagawa, H. Iida, and M. Sokabe
A Mechanosensitive Anion Channel in Arabidopsis thaliana Mesophyll Cells
Plant Cell Physiol., November 15, 2004; 45(11): 1704 - 1708.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. R. Lew
Osmotic Effects on the Electrical Properties of Arabidopsis Root Hair Vacuoles in Situ
Plant Physiology, January 1, 2004; 134(1): 352 - 360.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. S. Ober and R. E. Sharp
Electrophysiological responses of maize roots to low water potentials: relationship to growth and ABA accumulation
J. Exp. Bot., February 1, 2003; 54(383): 813 - 824.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2002 by the American Society of Plant Biologists