First published online April 9, 2002; 10.1104/pp.010869
Plant Physiol, May 2002, Vol. 129, pp. 50-63
Adaptation of H+-Pumping and Plasma Membrane
H+ ATPase Activity in Proteoid Roots of White Lupin under
Phosphate Deficiency1
Feng
Yan,*
Yiyong
Zhu,
Caroline
Müller,
Christian
Zörb, and
Sven
Schubert
Institute of Plant Nutrition, Interdisciplinary Research Center,
Justus Liebig University, Heinrich-Buff-Ring 26-32, D-35392 Giessen,
Germany
White lupin (Lupinus albus) is able to adapt
to phosphorus deficiency by producing proteoid roots that release a
huge amount of organic acids, resulting in mobilization of sparingly
soluble soil phosphate in rhizosphere. The mechanisms responsible for the release of organic acids by proteoid root cells, especially the
trans-membrane transport processes, have not been elucidated. Because
of high cytosolic pH, the release of undissociated organic acids is not
probable. In the present study, we focused on H+ export by
plasma membrane H+ ATPase in active proteoid roots. In
vivo, rhizosphere acidification of active proteoid roots was vanadate
sensitive. Plasma membranes were isolated from proteoid roots and
lateral roots from P-deficient and -sufficient plants. In vitro, in
comparison with two types of lateral roots and proteoid roots of
P-sufficient plants, the following increase of the various parameters
was induced in active proteoid roots of P-deficient plants: (a)
hydrolytic ATPase activity, (b) Vmax and
Km, (c) H+ ATPase enzyme
concentration of plasma membrane, (d) H+-pumping activity,
(e) pH gradient across the membrane of plasmalemma vesicles, and (f)
passive H+ permeability of plasma membrane. In addition,
lower vanadate sensitivity and more acidic pH optimum were determined
for plasma membrane ATPase of active proteoid roots. Our data support
the hypothesis that in active proteoid root cells, H+ and
organic anions are exported separately, and that modification of plasma
membrane H+ ATPase is essential for enhanced rhizosphere
acidification by active proteoid roots.
1
This work was supported by the German Science
Foundation (grant no. Ya 47/1-1).
*
Corresponding author; e-mail feng.yan{at}ernaehrung.unigiessen.de;
fax 49-641-99-39-169.
© 2002 American Society of Plant Physiologists
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