First published online March 22, 2002; 10.1104/pp.010829
Plant Physiol, May 2002, Vol. 129, pp. 85-94
Characterization of FRO1, a Pea Ferric-Chelate Reductase Involved
in Root Iron Acquisition1
Brian M.
Waters,
Dale G.
Blevins, and
David J.
Eide*
Departments of Agronomy (B.M.W., D.G.B.) and Nutritional Sciences
(D.J.E.), University of Missouri, Columbia, Missouri 65211
To acquire iron, many plant species reduce soil Fe(III) to Fe(II)
by Fe(III)-chelate reductases embedded in the plasma membrane of root
epidermal cells. The reduced product is then taken up by Fe(II)
transporter proteins. These activities are induced under Fe deficiency.
We describe here the FRO1 gene from pea (Pisum sativum), which encodes an Fe(III)-chelate reductase.
Consistent with this proposed role, FRO1 shows similarity to other
oxidoreductase proteins, and expression of FRO1 in yeast
conferred increased Fe(III)-chelate reductase activity. Furthermore,
FRO1 mRNA levels in plants correlated with
Fe(III)-chelate reductase activity. Sites of FRO1
expression in roots, leaves, and nodules were determined. FRO1 mRNA was detected throughout the root, but was most
abundant in the outer epidermal cells. Expression was detected in
mesophyll cells in leaves. In root nodules, mRNA was detected in the
infection zone and nitrogen-fixing region. These results indicate that
FRO1 acts in root Fe uptake and they suggest a role in Fe distribution throughout the plant. Characterization of FRO1 has also provided new
insights into the regulation of Fe uptake. FRO1
expression and reductase activity was detected only in Fe-deficient
roots of Sparkle, whereas both were constitutive in brz
and dgl, two mutants with incorrectly regulated Fe
accumulation. In contrast, FRO1 expression was
responsive to Fe status in shoots of all three plant lines. These
results indicate differential regulation of FRO1 in
roots and shoots, and improper FRO1 regulation in
response to a shoot-derived signal of iron status in the roots of the
brz and dgl mutants.
1
This work was supported by the Plant Science
Unit and by the National Institutes of Health (grant no.
1RO1-GM58265).
*
Corresponding author; e-mail eided{at}missouri.edu; fax
573-882-0185.
© 2002 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
E. Bacaicoa and J. M. Garcia-Mina
Iron Efficiency in Different Cucumber Cultivars: The Importance of Optimizing the Use of Foliar Iron
J. Amer. Soc. Hort. Sci.,
July 1, 2009;
134(4):
405 - 416.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Tsukamoto, H. Nakanishi, H. Uchida, S. Watanabe, S. Matsuhashi, S. Mori, and N. K. Nishizawa
52Fe Translocation in Barley as Monitored by a Positron-Emitting Tracer Imaging System (PETIS): Evidence for the Direct Translocation of Fe from Roots to Young Leaves via Phloem
Plant Cell Physiol.,
January 1, 2009;
50(1):
48 - 57.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Jeong, C. Cohu, L. Kerkeb, M. Pilon, E. L. Connolly, and M. L. Guerinot
Chloroplast Fe(III) chelate reductase activity is essential for seedling viability under iron limiting conditions
PNAS,
July 29, 2008;
105(30):
10619 - 10624.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Zocchi, P. De Nisi, M. Dell'Orto, L. Espen, and P. M. Gallina
Iron deficiency differently affects metabolic responses in soybean roots
J. Exp. Bot.,
March 1, 2007;
58(5):
993 - 1000.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Lucena, B. M. Waters, F. J. Romera, M. J. Garcia, M. Morales, E. Alcantara, and R. Perez-Vicente
Ethylene could influence ferric reductase, iron transporter, and H+-ATPase gene expression by affecting FER (or FER-like) gene activity
J. Exp. Bot.,
December 1, 2006;
57(15):
4145 - 4154.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. M. Waters, H.-H. Chu, R. J. DiDonato, L. A. Roberts, R. B. Eisley, B. Lahner, D. E. Salt, and E. L. Walker
Mutations in Arabidopsis Yellow Stripe-Like1 and Yellow Stripe-Like3 Reveal Their Roles in Metal Ion Homeostasis and Loading of Metal Ions in Seeds
Plant Physiology,
August 1, 2006;
141(4):
1446 - 1458.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Feng, F. An, S. Zhang, Z. Ji, H.-Q. Ling, and J. Zuo
Light-Regulated, Tissue-Specific, and Cell Differentiation-Specific Expression of the Arabidopsis Fe(III)-Chelate Reductase Gene AtFRO6
Plant Physiology,
April 1, 2006;
140(4):
1345 - 1354.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Wu, L. Li, J. Du, Y. Yuan, X. Cheng, and H.-Q. Ling
Molecular and Biochemical Characterization of the Fe(III) Chelate Reductase Gene Family in Arabidopsis thaliana
Plant Cell Physiol.,
September 1, 2005;
46(9):
1505 - 1514.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. L. Connolly, N. H. Campbell, N. Grotz, C. L. Prichard, and M. L. Guerinot
Overexpression of the FRO2 Ferric Chelate Reductase Confers Tolerance to Growth on Low Iron and Uncovers Posttranscriptional Control
Plant Physiology,
November 1, 2003;
133(3):
1102 - 1110.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H.-Q. Ling, P. Bauer, Z. Bereczky, B. Keller, and M. Ganal
The tomato fer gene encoding a bHLH protein controls iron-uptake responses in roots
PNAS,
October 15, 2002;
99(21):
13938 - 13943.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
