First published online April 9, 2002; 10.1104/pp.010948
Plant Physiol, May 2002, Vol. 129, pp. 95-102
Direct Interference with Rhamnogalacturonan I Biosynthesis in
Golgi Vesicles1
Michael
Skjøt,
Markus
Pauly,2
Maxwell S.
Bush,
Bernhard
Borkhardt,
Maureen C.
McCann, and
Peter
Ulvskov*
Biotechnology Group, Danish Institute of Agricultural Sciences,
Thorvaldsensvej 40, 1871 Copenhagen, Denmark (M.S., B.B., P.U.);
Department of Plant Biology, Plant Biochemistry Laboratory, The Royal
Veterinary and Agricultural University, Thorvaldsensvej 40, 1871 Copenhagen, Denmark (M.P.); and Department of Cell and Developmental
Biology, John Innes Centre, Norwich Research Park, Colney Lane, NR4 7UH
Norwich, United Kingdom (M.S.B., M.C.M.)
Pectin is a class of complex cell wall polysaccharides with
multiple roles during cell development. Assigning specific functions to
particular polysaccharides is in its infancy, in part, because of the
limited number of mutants and transformants available with modified
pectic polymers in their walls. Pectins are also important polymers
with diverse applications in the food and pharmaceutical industries,
which would benefit from technology for producing pectins with specific
functional properties. In this report, we describe the generation of
potato (Solanum tuberosum L. cv Posmo) tuber
transformants producing pectic rhamnogalacturonan I (RGI) with a low
level of arabinosylation. This was achieved by the expression of a
Golgi membrane-anchored endo- -1,5-arabinanase. Sugar composition
analysis of RGI isolated from transformed and wild-type tubers showed
that the arabinose content was decreased by approximately 70% in
transformed cell walls compared with wild type. The modification of the
RGI was confirmed by immunolabeling with an antibody recognizing
-1,5-arabinan. This is the first time, to our knowledge, that the
biosynthesis of a plant cell wall polysaccharide has been manipulated
through the action of a glycosyl hydrolase targeted to the Golgi compartment.
1
This work was supported by the Danish Research
Council's Technology by Highly Oriented Research program, by
The Danish National Research Foundation, by the European Commission
(grant no. BIOTECH CT97-2224), and by a Royal Society University
Research Fellowship (to M.C.M.).
*
Corresponding author; e-mail p.ulvskov{at}dias.kvl.dk; fax
45-3528-2581.
2
Present address: Max-Planck-Institute for Molecular
Plant Physiology, Am Muehlenberg 1, 14476 Golm, Germany.
© 2002 American Society of Plant Physiologists
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