First published online May 2, 2002; 10.1104/pp.003970
Plant Physiol, June 2002, Vol. 129, pp. 530-539
NPSN11 Is a Cell Plate-Associated SNARE Protein That Interacts
with the Syntaxin KNOLLE1
Haiyan
Zheng,2
Sebastian Y.
Bednarek,
Anton A.
Sanderfoot,
Jose
Alonso,
Joseph R.
Ecker, and
Natasha V.
Raikhel*
Department of Energy Plant Research Laboratory, Michigan
State University, East Lansing, Michigan 48824-1312 (H.Z., A.A.S.,
N.V.R.); Department of Biochemistry, University of Wisconsin-Madison,
Madison, Wisconsin 53706-1544 (S.Y.B.); Plant Biology Laboratory Salk
Institute Genomic Analysis Laboratory (SIGnAL), The Salk Institute for
Biological Studies, 10010 North Torrey Pines Road, La Jolla, California
92037; and Department of Botany and Plant Sciences and The Center for
Plant Cell Biology, 2109 Batchelor Hall, University of California,
Riverside, California 92521 (N.V.R.)
SNAREs are important components of the vesicle
trafficking machinery in eukaryotic cells. In plants, SNAREs have been
found to play a variety of roles in the development and physiology of the whole organism. Here, we describe the identification and
characterization of a novel plant-specific SNARE, NPSN11, a member of a
closely related small gene family in Arabidopsis. NSPN11 is highly
expressed in actively dividing cells. In a subcellular fractionation
experiment, NSPN11 cofractionates with the cytokinesis-specific
syntaxin, KNOLLE, which is required for the formation of the cell
plate. By immunofluorescence microscopy, NSPN11 was localized to the cell plate in dividing cells. Consistent with the localization studies,
NSPN11 was found to interact with KNOLLE. Our results suggest that
NPSN11 is another component of the membrane trafficking and fusion
machinery involved in cell plate formation.
1
This research was supported by the National
Science Foundation grant no. MCB-0296080 (to N.V.R.) and by the
U.S. Department of Energy, Division of Energy Biosciences (project no.
DE-FG02-99ER203 32 to S.Y.B.).
2
Present address: Core Technology Area, Discovery
Research, Novartis Pharmaceuticals Corporation, 556 Morris Avenue,
Summit, NJ 07901.
*
Corresponding author; e-mail natasha.raikhel{at}ucr.edu; fax
909-787-4437.
http://www.cepceb.ucr.edu/members/raikhel.htm.
© 2002 American Society of Plant Physiologists
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