First published online May 2, 2002; 10.1104/pp.002725
Plant Physiol, June 2002, Vol. 129, pp. 594-604
The pgp1 Mutant Locus of Arabidopsis Encodes a
Phosphatidylglycerolphosphate Synthase with Impaired
Activity1
Changcheng
Xu,2
Heiko
Härtel,23
Hajime
Wada,
Miki
Hagio,
Bin
Yu,
Chris
Eakin, and
Christoph
Benning*
Department of Biochemistry and Molecular Biology, Michigan State
University, East Lansing, Michigan 48824-1319 (C.X., H.H., B.Y., C.E.,
C.B.); and Department of Biology, Faculty of Sciences, Kyushu
University, Ropponmatsu, Fukuoka 810-8560, Japan (H.W., M.H.)
Phosphatidylglycerol is a ubiquitous phospholipid that is
also present in the photosynthetic membranes of plants. Multiple independent lines of evidence suggest that this lipid plays a critical
role for the proper function of photosynthetic membranes and cold
acclimation. In eukaryotes, different subcellular compartments are
competent for the biosynthesis of phosphatidylglycerol. Details on the
plant-specific pathways in different organelles are scarce. Here, we
describe a phosphatidylglycerol biosynthesis-deficient mutant of
Arabidopsis, pgp1. The overall content of
phosphatidylglycerol is reduced by 30%. This mutant carries a point
mutation in the CDP-alcohol phosphotransferase motif of the
phosphatidylglycerolphosphate synthase (EC 2.7.8.5) isoform encoded by
a gene on chromosome 2. The mutant shows an 80% reduction in plastidic
phosphatidylglycerolphosphate synthase activity consistent with the
plastidic location of this particular isoform. Mutant plants are pale
green, and their photosynthesis is impaired. This mutant provides a
promising new tool to elucidate the biosynthesis and function of
plastidic phosphatidylglycerol in seed plants.
1
This work was supported by the U.S. Department
of Energy (grant no. DE-FG02-98ER20305 to C.B.), by the Michigan
State University Center for Novel Plant Products (grant to C.B.), by a
Grant-in-Aid for Scientific Research from the Japanese Ministry of
Education, Science, Sport and Culture (grant no. 12640635 to H.W.), and
by a Program for Promotion of Basic Research Activities for
Innovative Biosciences grant (to H.W.) from the Bio-oriented Technology
Research Advancement Institution (Tokyo). M.H. was supported by a
fellowship from the Japanese Society for the Promotion of Science.
2
These authors contributed equally to the paper.
3
Present address: BASF Plant Science LLC, P.O. Box 13528, 26 Davis Drive, Research Triangle Park, NC 27709-3528.
*
Corresponding author; e-mail benning{at}msu.edu; fax
517-353-9334.
© 2002 American Society of Plant Physiologists
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