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First published online May 2, 2002; 10.1104/pp.002725

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Plant Physiol, June 2002, Vol. 129, pp. 594-604

The pgp1 Mutant Locus of Arabidopsis Encodes a Phosphatidylglycerolphosphate Synthase with Impaired Activity1

Changcheng Xu,2 Heiko Härtel,23 Hajime Wada, Miki Hagio, Bin Yu, Chris Eakin, and Christoph Benning*

Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824-1319 (C.X., H.H., B.Y., C.E., C.B.); and Department of Biology, Faculty of Sciences, Kyushu University, Ropponmatsu, Fukuoka 810-8560, Japan (H.W., M.H.)

Phosphatidylglycerol is a ubiquitous phospholipid that is also present in the photosynthetic membranes of plants. Multiple independent lines of evidence suggest that this lipid plays a critical role for the proper function of photosynthetic membranes and cold acclimation. In eukaryotes, different subcellular compartments are competent for the biosynthesis of phosphatidylglycerol. Details on the plant-specific pathways in different organelles are scarce. Here, we describe a phosphatidylglycerol biosynthesis-deficient mutant of Arabidopsis, pgp1. The overall content of phosphatidylglycerol is reduced by 30%. This mutant carries a point mutation in the CDP-alcohol phosphotransferase motif of the phosphatidylglycerolphosphate synthase (EC 2.7.8.5) isoform encoded by a gene on chromosome 2. The mutant shows an 80% reduction in plastidic phosphatidylglycerolphosphate synthase activity consistent with the plastidic location of this particular isoform. Mutant plants are pale green, and their photosynthesis is impaired. This mutant provides a promising new tool to elucidate the biosynthesis and function of plastidic phosphatidylglycerol in seed plants.

1 This work was supported by the U.S. Department of Energy (grant no. DE-FG02-98ER20305 to C.B.), by the Michigan State University Center for Novel Plant Products (grant to C.B.), by a Grant-in-Aid for Scientific Research from the Japanese Ministry of Education, Science, Sport and Culture (grant no. 12640635 to H.W.), and by a Program for Promotion of Basic Research Activities for Innovative Biosciences grant (to H.W.) from the Bio-oriented Technology Research Advancement Institution (Tokyo). M.H. was supported by a fellowship from the Japanese Society for the Promotion of Science.

2 These authors contributed equally to the paper.

3 Present address: BASF Plant Science LLC, P.O. Box 13528, 26 Davis Drive, Research Triangle Park, NC 27709-3528.

* Corresponding author; e-mail benning{at}msu.edu; fax 517-353-9334.

© 2002 American Society of Plant Physiologists


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