First published online May 24, 2002; 10.1104/pp.020002
Plant Physiol, June 2002, Vol. 129, pp. 605-615
SPINDLY Is a Nuclear-Localized Repressor of Gibberellin Signal
Transduction Expressed throughout the Plant1
Stephen M.
Swain,*
Tong-Seung
Tseng,
Tina M.
Thornton,
Manjula
Gopalraj, and
Neil E.
Olszewski
Department of Plant Biology and Plant Molecular Genetics Institute,
University of Minnesota, St. Paul, Minnesota 55108
SPY (SPINDLY) encodes a
putative O-linked N-acetyl-glucosamine
transferase that is genetically defined as a negatively acting component of the gibberellin (GA) signal transduction pathway. Analysis
of Arabidopsis plants containing a
SPY::GUS reporter gene reveals that
SPY is expressed throughout the life of the plant and in
most plant organs examined. In addition to being expressed in all
organs where phenotypes due to spy mutations have been
reported, SPY::GUS is expressed in the root.
Examination of the roots of wild-type, spy, and
gai plants revealed phenotypes indicating that SPY and
GAI play a role in root development. A second
SPY::GUS reporter gene lacking part of the SPY
promoter was inactive, suggesting that sequences in the first exon
and/or intron are required for detectable expression. Using both
subcellular fractionation and visualization of a SPY-green fluorescent
protein fusion protein that is able to rescue the
spy mutant phenotype, the majority of SPY protein was
shown to be present in the nucleus. This result is consistent with the
nuclear localization of other components of the GA response pathway and
suggests that SPY's role as a negative regulator of GA signaling
involves interaction with other nuclear proteins and/or
O-N-acetyl-glucosamine modification of these proteins.
1
This work was supported by the National Science
Foundation (grant nos. MCB-9604126 and MCB-9983583 to
N.E.O.).
*
Corresponding author; e-mail Steve.Swain{at}csiro.au; fax
61-3-50513111.
© 2002 American Society of Plant Physiologists
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