First published online May 24, 2002; 10.1104/pp.004184
Plant Physiol, June 2002, Vol. 129, pp. 678-690
Cytokinesis-Defective Mutants of Arabidopsis1
Rosi
Söllner,
Gerti
Glässer,
Gehard
Wanner,
Chris R.
Somerville,
Gerd
Jürgens, and
Farhah F.
Assaad*
Genetics and Microbiology Institute, Ludwig Maximillian University,
Maria Ward Strasse 1a, 80638 Munich, Germany (R.S., G.G., F.F.A.);
Botany Institute, Ludwig Maximillian University, Menzingerstrasse 67, 80638 Munich, Germany (G.W.); Carnegie Institution, Department of Plant
Biology, 260 Panama Street, Stanford, California 94305 (C.R.S.); and
Lehrstuhl für Entwicklungsgenetik, Universität
Tübingen, D-72076 Tübingen, Germany (G.J.)
We have identified mutations in six previously
uncharacterized genes of Arabidopsis, named club,
bublina, massue, rod,
bloated, and bims, that are required for
cytokinesis. The mutants are seedling lethal, have morphological
abnormalities, and are characterized by cell wall stubs, gapped walls,
and multinucleate cells. In these and other respects, the new mutants
are phenotypically similar to knolle,
keule, hinkel, and pleiade
mutants. The mutants display a gradient of stomatal phenotypes,
correlating roughly with the severity of their cytokinesis defect.
Similarly, the extent to which the different mutant lines were capable
of growing in tissue culture correlated well with the severity of the
cytokinesis defect. Phenotypic analysis of the novel and previously
characterized loci indicated that the secondary consequences of a
primary defect in cytokinesis include anomalies in body organization,
organ number, and cellular differentiation, as well as organ fusions
and perturbations of the nuclear cycle. Two of the 10 loci are required
for both cytokinesis and root hair morphogenesis. The results have
implications for the identification of novel cytokinesis genes and
highlight the mechanistic similarity between cytokinesis and root hair
morphogenesis, two processes that result in a rapid deposition of new
cell walls via polarized secretion.
1
This work was supported by a European Union
Biotechnology Program Framework IV grant (to G.J.), by the U.S.
Department of Energy (grant no. DOE-FG02-00ER20133 to C.R.S.), and by
the Deutsche Forschungsgemeinschaft (grant no. AS110/2-1 to F.F.A.).
F.F.A. was supported by a long-term European Molecular Biology
Organization fellowship and a Hochschulsonderprogram stipend
from the University of Munich.
*
Corresponding author; e-mail fassaad{at}andrew2.stanford.edu; fax
650-325-6857.
© 2002 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
D. Van Damme, D. Inze, and E. Russinova
Vesicle Trafficking during Somatic Cytokinesis
Plant Physiology,
August 1, 2008;
147(4):
1544 - 1552.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. M. Castellano and R. Sablowski
Phosducin-Like Protein 3 Is Required for Microtubule-Dependent Steps of Cell Division but Not for Meristem Growth in Arabidopsis
PLANT CELL,
April 1, 2008;
20(4):
969 - 981.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-M. Chow, H. Neto, C. Foucart, and I. Moore
Rab-A2 and Rab-A3 GTPases Define a trans-Golgi Endosomal Membrane Domain in Arabidopsis That Contributes Substantially to the Cell Plate
PLANT CELL,
January 1, 2008;
20(1):
101 - 123.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. E. Griffith, U. Mayer, A. Capron, Q. A. Ngo, A. Surendrarao, R. McClinton, G. Jurgens, and V. Sundaresan
The TORMOZ Gene Encodes a Nucleolar Protein Required for Regulated Division Planes and Embryo Development in Arabidopsis
PLANT CELL,
July 1, 2007;
19(7):
2246 - 2263.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Haga, K. Kato, M. Murase, S. Araki, M. Kubo, T. Demura, K. Suzuki, I. Muller, U. Voss, G. Jurgens, et al.
R1R2R3-Myb proteins positively regulate cytokinesis through activation of KNOLLE transcription in Arabidopsis thaliana
Development,
March 15, 2007;
134(6):
1101 - 1110.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Kawamura, R. Himmelspach, M. C. Rashbrooke, A. T. Whittington, K. R. Gale, D. A. Collings, and G. O. Wasteneys
MICROTUBULE ORGANIZATION 1 Regulates Structure and Function of Microtubule Arrays during Mitosis and Cytokinesis in the Arabidopsis Root
Plant Physiology,
January 1, 2006;
140(1):
102 - 114.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. CONSONNI, G. GAVAZZI, and S. DOLFINI
Genetic Analysis as a Tool to Investigate the Molecular Mechanisms Underlying Seed Development in Maize
Ann. Bot.,
September 1, 2005;
96(3):
353 - 362.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Favery, L. A. Chelysheva, M. Lebris, F. Jammes, A. Marmagne, J. de Almeida-Engler, P. Lecomte, C. Vaury, R. A. Arkowitz, and P. Abad
Arabidopsis Formin AtFH6 Is a Plasma Membrane-Associated Protein Upregulated in Giant Cells Induced by Parasitic Nematodes
PLANT CELL,
September 1, 2004;
16(9):
2529 - 2540.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. P. Smertenko, H.-Y. Chang, V. Wagner, D. Kaloriti, S. Fenyk, S. Sonobe, C. Lloyd, M.-T. Hauser, and P. J. Hussey
The Arabidopsis Microtubule-Associated Protein AtMAP65-1: Molecular Analysis of Its Microtubule Bundling Activity
PLANT CELL,
August 1, 2004;
16(8):
2035 - 2047.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Segui-Simarro, J. R. Austin II, E. A. White, and L. A. Staehelin
Electron Tomographic Analysis of Somatic Cell Plate Formation in Meristematic Cells of Arabidopsis Preserved by High-Pressure Freezing
PLANT CELL,
April 1, 2004;
16(4):
836 - 856.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Boudolf, R. Barroco, J. d. A. Engler, A. Verkest, T. Beeckman, M. Naudts, D. Inze, and L. De Veylder
B1-Type Cyclin-Dependent Kinases Are Essential for the Formation of Stomatal Complexes in Arabidopsis thaliana
PLANT CELL,
April 1, 2004;
16(4):
945 - 955.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Costa, J. F. Gutierrez-Marcos, T. P. Brutnell, A. J. Greenland, and H. G. Dickinson
The globby1-1 (glo1-1) mutation disrupts nuclear and cell division in the developing maize seed causing alterations in endosperm cell fate and tissue differentiation
Development,
October 15, 2003;
130(20):
5009 - 5017.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. G. Falbel, L. M. Koch, J. A. Nadeau, J. M. Segui-Simarro, F. D. Sack, and S. Y. Bednarek
SCD1 is required for cell cytokinesis and polarized cell expansion in Arabidopsis thaliana
Development,
September 1, 2003;
130(17):
4011 - 4024.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B.-H. Kang, J. S. Busse, and S. Y. Bednarek
Members of the Arabidopsis Dynamin-Like Gene Family, ADL1, Are Essential for Plant Cytokinesis and Polarized Cell Growth
PLANT CELL,
April 1, 2003;
15(4):
899 - 913.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
