Plant Physiol, June 2002, Vol. 129, pp. 691-705
RASPBERRY3 Gene Encodes a Novel Protein Important
for Embryo Development
Nestor R.
Apuya,1
Ramin
Yadegari,2
Robert
L.
Fischer,
John J.
Harada, and
Robert B.
Goldberg*
Department of Molecular, Cell, and Developmental Biology,
University of California, Los Angeles, California 90095-1606 (N.R.A.,
R.Y., R.B.G.); Department of Plant and Microbial Biology, University of
California, Berkeley, California 94720 (R.L.F.); and Section of Plant
Biology, Division of Biological Sciences, University of California,
Davis, California 95616 (J.H.H.)
We identified a new gene that is interrupted by T-DNA in an
Arabidopsis embryo mutant called raspberry3.
raspberry3 has "raspberry-like" cellular
protuberances with an enlarged suspensor characteristic of other
raspberry embryo mutants, and is arrested
morphologically at the globular stage of embryo development. The
predicted RASPBERRY3 protein has domains found in proteins present in
prokaryotes and algae chloroplasts. Computer prediction analysis
suggests that the RASPBERRY3protein may be localized in the
chloroplast. Complementation analysis supports the possibility that the
RASPBERRY3 protein may be involved in chloroplast development. Our
experiments demonstrate the important role of the chloroplast, directly
or indirectly, in embryo morphogenesis and development.
1
Present address: Ceres Inc., 3007 Malibu Canyon,
Malibu, CA 90265.
2
Present address: Department of Plant Sciences,
University of Arizona, Forbes Building 303, P.O. Box 210036, Tucson, AZ 85721-0036.
*
Corresponding author; e-mail bobg{at}ucla.edu; fax
310-825-8201.
© 2002 American Society of Plant Physiologists
