First published online May 2, 2002; 10.1104/pp.001057
Plant Physiol, June 2002, Vol. 129, pp. 706-716
Potentiation of Developmentally Regulated Plant Defense Response
by AtWRKY18, a Pathogen-Induced Arabidopsis Transcription
Factor1
Chunhong
Chen and
Zhixiang
Chen*
Department of Microbiology, Molecular Biology, and Biochemistry,
University of Idaho, Moscow, Idaho 83844-3052
AtWRKY18 is a pathogen- and salicylic acid-induced
Arabidopsis transcription factor containing the plant-specific WRKY
zinc finger DNA-binding motif. In the present study, we have
transformed Arabidopsis plants with AtWRKY18
under control of the cauliflower mosaic virus 35S promoter.
Surprisingly, transgenic plants expressing high levels of
AtWRKY18 were stunted in growth. When expressed at
moderate levels, AtWRKY18 potentiated developmentally regulated defense
responses in transgenic plants without causing substantial negative
effects on plant growth. As they grew from seedling to mature stages,
transgenic AtWRKY18 plant showed marked increase in the
expression of pathogenesis-related genes and resistance to the
bacterial pathogen Pseudomonas syringae, whereas
wild-type plants exhibited little enhancement in these defense
responses. Potentiation of developmentally regulated defense responses
by AtWRKY18 was not associated with enhanced biosynthesis of salicylic acid but required the disease resistance regulatory protein NPR1/NIM1. Thus, AtWRKY18 can positively modulate defense-related gene
expression and disease resistance. To study the regulated expression of
AtWRKY18, we have identified a cluster of WRKY binding
sites in the promoter of the gene and demonstrated that they acted as
negative regulatory elements for the inducible expression of
AtWRKY18. These negative cis-acting elements may prevent
overexpression of AtWRKY18 during the activation of
plant defense responses that could be detrimental to plant growth as
inferred from the transgenic plants ectopically expressing the transgene.
1
This work was supported in part by the National
Science Foundation (grant no. MCB-9905976 to Z.C.).
*
Corresponding author; e-mail zchen{at}uidaho.edu; fax
208-885-6518.
© 2002 American Society of Plant Physiologists
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