First published online May 24, 2002; 10.1104/pp.005280
Plant Physiol, June 2002, Vol. 129, pp. 886-896
Analysis of the NRT2 Nitrate Transporter Family in Arabidopsis.
Structure and Gene Expression
Mathilde
Orsel,
Anne
Krapp, and
Françoise
Daniel-Vedele*
Unité de la Nutrition Azotée des Plantes, Institut
National de la Recherche Agronomique, Route de St. Cyr, F-78026
Versailles cedex, France
Nitrate is an essential element for plant growth, both as a
primary nutrient in the nitrogen assimilation pathway and as an important signal for plant development. The uptake of nitrate from the
soil and its translocation throughout the plant has been the subject of
intensive physiological and molecular studies. Using a reverse genetic
approach, the AtNRT2.1 gene has been shown to be
involved in the inducible component of the high-affinity nitrate
transport system in Arabidopsis. The Arabidopsis Genome Initiative has
released nearly the whole genome sequence of Arabidopsis, allowing the
identification of a small NRT2 multigene family in this species. Thus,
we investigated the phylogenetic relationship between NRT2 proteins
belonging to several kingdoms and compared the structure of the
different members of the Arabidopsis family. We analyzed, by
semiquantitative reverse transcriptase-polymerase chain reaction, the
expression pattern of each gene depending on plant organ and
development or nutritional status, and compared the relative level of
each gene by real-time polymerase chain reaction. We also evaluated the
significance of each paralog on the basis of the relative levels of
gene expression. The results are discussed in relation with distinct
roles for the individual members of the AtNRT2 family.
*
Corresponding author; e-mail vedele{at}versailles.inra.fr; fax
0-1-30-83-30-96.
© 2002 American Society of Plant Physiologists
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