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Plant Physiol, July 2002, Vol. 129, pp. 1003-1018
Methyl Jasmonate Induces Traumatic Resin Ducts, Terpenoid Resin
Biosynthesis, and Terpenoid Accumulation in Developing Xylem of Norway
Spruce Stems1
Diane
Martin,
Dorothea
Tholl,
Jonathan
Gershenzon, and
Jörg
Bohlmann*
Max Planck Institute for Chemical Ecology, Carl Zeiss Promenade 10, 07745 Jena, Germany (D.M., D.T., J.G., J.B.); and Biotechnology
Laboratory (D.M., J.B.), Department of Botany (D.M., J.B.), and
Department of Forest Sciences (J.B.), University of British Columbia,
6174 University Boulevard, Vancouver, Canada V6T 1Z3
Norway spruce (Picea abies L. Karst) produces an
oleoresin characterized by a diverse array of terpenoids,
monoterpenoids, sesquiterpenoids, and diterpene resin acids that can
protect conifers against potential herbivores and pathogens. Oleoresin
accumulates constitutively in resin ducts in the cortex and phloem
(bark) of Norway spruce stems. De novo formation of traumatic resin
ducts (TDs) is observed in the developing secondary xylem (wood) after insect attack, fungal elicitation, and mechanical wounding. Here, we
characterize the methyl jasmonate-induced formation of TDs in Norway
spruce by microscopy, chemical analyses of resin composition, and
assays of terpenoid biosynthetic enzymes. The response involves tissue-specific differentiation of TDs, terpenoid accumulation, and
induction of enzyme activities of both prenyltransferases and terpene
synthases in the developing xylem, a tissue that constitutively lacks
axial resin ducts in spruce. The induction of a complex defense
response in Norway spruce by methyl jasmonate application provides new
avenues to evaluate the role of resin defenses for protection of
conifers against destructive pests such as white pine weevils
(Pissodes strobi), bark beetles (Coleoptera,
Scolytidae), and insect-associated tree pathogens.
1
This work was supported by the Natural Sciences
and Engineering Research Council (funds to J.B.), by the Canadian
Foundation for Innovation (funds to J.B.), by the British Columbia
Ministry of Forests (funds to J.B.), and by the Max Planck Society
(funds to J.G. and fellowships to D.M. and D.T.).
*
Corresponding author; e-mail bohlmann{at}interchange.ubc.ca; fax
604-822-6097.
© 2002 American Society of Plant Physiologists
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