First published online June 14, 2002; 10.1104/pp.003681
Plant Physiol, July 2002, Vol. 129, pp. 1308-1319
Proteome Analysis of Grain Filling and Seed Maturation in
Barley1
Christine
Finnie,
Sabrina
Melchior,
Peter
Roepstorff, and
Birte
Svensson*
Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej
10, DK-2500 Valby, Copenhagen, Denmark (C.F., B.S.); and Department of
Biochemistry and Molecular Biology, University of Southern Denmark,
Campusvej 55, DK-5230 Odense M, Denmark (S.M., P.R.)
In monocotyledonous plants, the process of seed development
involves the deposition of reserves in the starchy endosperm and development of the embryo and aleurone layer. The final stages of seed
development are accompanied by an increase in desiccation tolerance and
drying out of the mature seed. We have used two-dimensional gel
electrophoresis for a time-resolved study of the changes in proteins
that occur during seed development in barley (Hordeum vulgare). About 1,000 low-salt extractable protein spots could be resolved on the two-dimensional gels. Protein spots were divided into six categories according to the timing of appearance or
disappearance during the 5-week period of comparison. Nineteen
different proteins or protein fragments in 36 selected spots were
identified by matrix-assisted laser-desorption ionization time
of flight mass spectrometry (MS) or nano-electrospray tandem MS/MS.
Some proteins were present throughout development (for example,
cytosolic malate dehydrogenase), whereas others were associated with
the early grain filling (ascorbate peroxidase) or desiccation (Cor14b)
stages. Most noticeably, the development process is characterized by an
accumulation of low-Mr  -amylase/trypsin
inhibitors, serine protease inhibitors, and enzymes involved in
protection against oxidative stress. We present examples of proteins
not previously experimentally observed, differential extractability of
thiol-bound proteins, and possible allele-specific spot variation. Our
results both confirm and expand on knowledge gained from previous
analyses of individual proteins involved in grain filling and maturation.
1
This work was supported by the Danish Research
Agency samarbejde mellem sektorforskning universiteter og erhverv (SUE)
program (grant no. 9901194).
*
Corresponding author; e-mail bis{at}crc.dk; fax
45-33-27-47-08.
© 2002 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
T. Abebe, R. P. Wise, and R. W. Skadsen
Comparative Transcriptional Profiling Established the Awn as the Major Photosynthetic Organ of the Barley Spike While the Lemma and the Palea Primarily Protect the Seed
The Plant Genome,
November 1, 2009;
2(3):
247 - 259.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Shahpiri, B. Svensson, and C. Finnie
From Proteomics to Structural Studies of Cytosolic/Mitochondrial-Type Thioredoxin Systems in Barley Seeds
Mol Plant,
May 1, 2009;
2(3):
378 - 389.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Shahpiri, B. Svensson, and C. Finnie
The NADPH-Dependent Thioredoxin Reductase/Thioredoxin System in Germinating Barley Seeds: Gene Expression, Protein Profiles, and Interactions between Isoforms of Thioredoxin h and Thioredoxin Reductase
Plant Physiology,
February 1, 2008;
146(2):
789 - 799.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Mechin, C. Thevenot, M. Le Guilloux, J.-L. Prioul, and C. Damerval
Developmental Analysis of Maize Endosperm Proteome Suggests a Pivotal Role for Pyruvate Orthophosphate Dikinase
Plant Physiology,
March 1, 2007;
143(3):
1203 - 1219.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Fujiwara, K. Umemura, T. Kawasaki, and K. Shimamoto
Proteomics of Rac GTPase Signaling Reveals Its Predominant Role in Elicitor-Induced Defense Response of Cultured Rice Cells
Plant Physiology,
February 1, 2006;
140(2):
734 - 745.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Gallardo, C. Le Signor, J. Vandekerckhove, R. D. Thompson, and J. Burstin
Proteomics of Medicago truncatula Seed Development Establishes the Time Frame of Diverse Metabolic Processes Related to Reserve Accumulation
Plant Physiology,
October 1, 2003;
133(2):
664 - 682.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Triboi, P. Martre, and A.-M. Triboi-Blondel
Environmentally-induced changes in protein composition in developing grains of wheat are related to changes in total protein content
J. Exp. Bot.,
July 1, 2003;
54(388):
1731 - 1742.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
