First published online June 20, 2002; 10.1104/pp.001925
Plant Physiol, July 2002, Vol. 129, pp. 1368-1381
Cold-Regulated Cereal Chloroplast Late Embryogenesis
Abundant-Like Proteins. Molecular Characterization and Functional
Analyses
Christian
NDong,
Jean
Danyluk,
Kenneth E.
Wilson,
Tessa
Pocock,
Norman P.A.
Huner, and
Fathey
Sarhan*
Département des Sciences biologiques, Université du
Québec à Montréal, C.P. 8888 Succursale Centre-ville,
Montréal, Québec, Canada H3C 3P8 (C.N., J.D., F.S.);
Department of Molecular Biology, University of Geneva, 30 Quai
Ernest-Ansermet, CH-1211 Geneva 4, Switzerland (K.E.W.); and
Department of Plant Sciences, University of Western Ontario, London,
Ontario, Canada N6A 5B7 (T.P., N.P.A.H.)
Cold acclimation and freezing tolerance are the result of
complex interaction between low temperature, light, and photosystem II
(PSII) excitation pressure. Previous results have shown that expression
of the Wcs19 gene is correlated with PSII excitation pressure measured in vivo as the relative reduction state of PSII. Using cDNA library screening and data mining, we have identified three
different groups of proteins, late embryogenesis abundant (LEA) 3-L1,
LEA3-L2, and LEA3-L3, sharing identities with WCS19. These groups
represent a new class of proteins in cereals related to group 3 LEA
proteins. They share important characteristics such as a sorting signal
that is predicted to target them to either the chloroplast or
mitochondria and a C-terminal sequence that may be involved in
oligomerization. The results of subcellular fractionation,
immunolocalization by electron microscopy and the analyses of target
sequences within the Wcs19 gene are consistent with the
localization of WCS19 within the chloroplast stroma of wheat
(Triticum aestivum) and rye (Secale
cereale). Western analysis showed that the accumulation of
chloroplastic LEA3-L2 proteins is correlated with the capacity of
different wheat and rye cultivars to develop freezing tolerance.
Arabidopsis was transformed with the Wcs19 gene and the
transgenic plants showed a significant increase in their freezing
tolerance. This increase was only evident in cold-acclimated plants.
The putative function of this protein in the enhancement of freezing
tolerance is discussed.
*
Corresponding author; e-mail sarhan.fathey{at}uqam.ca; fax
514-987-4647.
© 2002 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
X. Sun, C. Hu, Q. Tan, J. Liu, and H. Liu
Effects of molybdenum on expression of cold-responsive genes in abscisic acid (ABA)-dependent and ABA-independent pathways in winter wheat under low-temperature stress
Ann. Bot.,
August 1, 2009;
104(2):
345 - 356.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Pacheco, C. Pereira, M. J. Almeida, and M. J. Sousa
Small heat-shock protein Hsp12 contributes to yeast tolerance to freezing stress
Microbiology,
June 1, 2009;
155(6):
2021 - 2028.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Battaglia, Y. Olvera-Carrillo, A. Garciarrubio, F. Campos, and A. A. Covarrubias
The Enigmatic LEA Proteins and Other Hydrophilins
Plant Physiology,
September 1, 2008;
148(1):
6 - 24.
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-Y. Yang, Y.-C. Chen, G. Y. Jauh, and C.-S. Wang
A Lily ASR Protein Involves Abscisic Acid Signaling and Confers Drought and Salt Resistance in Arabidopsis
Plant Physiology,
October 1, 2005;
139(2):
836 - 846.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Grelet, A. Benamar, E. Teyssier, M.-H. Avelange-Macherel, D. Grunwald, and D. Macherel
Identification in Pea Seed Mitochondria of a Late-Embryogenesis Abundant Protein Able to Protect Enzymes from Drying
Plant Physiology,
January 1, 2005;
137(1):
157 - 167.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-A. Lopez-Matas, P. Nunez, A. Soto, I. Allona, R. Casado, C. Collada, M.-A. Guevara, C. Aragoncillo, and L. Gomez
Protein Cryoprotective Activity of a Cytosolic Small Heat Shock Protein That Accumulates Constitutively in Chestnut Stems and Is Up-Regulated by Low and High Temperatures
Plant Physiology,
April 1, 2004;
134(4):
1708 - 1717.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. I. Watkinson, A. A. Sioson, C. Vasquez-Robinet, M. Shukla, D. Kumar, M. Ellis, L. S. Heath, N. Ramakrishnan, B. Chevone, L. T. Watson, et al.
Photosynthetic Acclimation Is Reflected in Specific Patterns of Gene Expression in Drought-Stressed Loblolly Pine
Plant Physiology,
December 1, 2003;
133(4):
1702 - 1716.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. Takumi, A. Koike, M. Nakata, S. Kume, R. Ohno, and C. Nakamura
Cold-specific and light-stimulated expression of a wheat (Triticum aestivum L.) Cor gene Wcor15 encoding a chloroplast-targeted protein
J. Exp. Bot.,
October 1, 2003;
54(391):
2265 - 2274.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Goyal, L. Tisi, A. Basran, J. Browne, A. Burnell, J. Zurdo, and A. Tunnacliffe
Transition from Natively Unfolded to Folded State Induced by Desiccation in an Anhydrobiotic Nematode Protein
J. Biol. Chem.,
April 4, 2003;
278(15):
12977 - 12984.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
