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Plant Physiol, July 2002, Vol. 129, pp. 931-942

Spectral Profiling for the Simultaneous Observation of Four Distinct Fluorescent Proteins and Detection of Protein-Protein Interaction via Fluorescence Resonance Energy Transfer in Tobacco Leaf Nuclei1

Naohiro Kato, Dominique Pontier,2 and Eric Lam*

Biotech Center, Rutgers University, 59 Dudley Road, New Brunswick, New Jersey 08901-8520

The control of subcellular localization of proteins and their interaction with other partners in vivo are important parameters that provide clues to their function and regulation. The ability to simultaneously track multiple protein species with high resolution should provide a valuable assay system to study and characterize various types of posttranslational control pathways. In this work, we established the system and a method involving "spectral profiling" for the resolution of four different fluorescent protein tags in the same viewing field using digital imaging technology. With these techniques, we have (a) developed new derivatives of mGFP5, which is commonly used in the plant field, that are about three times brighter; (b) demonstrated that four spectrally distinct fluorescent proteins (cyan, green, yellow, and red) that are fused to a transcription factor could be stably expressed in nuclei and distinguished in tobacco (Nicotiana tabacum) mesophyll cells; and (c) shown that interaction between partners of a dimeric transcription factor can be detected by measuring fluorescence resonance energy transfer. These technologies should help one to study protein-protein interactions efficiently, especially for nuclear proteins under in vivo conditions.

1 This work was supported by the Plant Genome Research Program of the National Science Foundation (grant nos. 9872636 and 0077167) and in part by the Charles and Johanna Busch Memorial Fund at Rutgers University (grant no. 6-49142).

2 Present address: Laboratoire de Génétique Moléculaire des Plantes, Université J. Fourier et Centre National de la Recherche Scientifique (Unité Mixte de Recherche 5575), Boite Postale 53, 38041 Grenoble cedex 9, France.

* Corresponding author; e-mail Lam{at}aesop.rutgers.edu; fax 732-932-6535.

© 2002 American Society of Plant Physiologists


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