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Plant Physiol, July 2002, Vol. 129, pp. 943-948
Ethanol Vapor Is an Efficient Inducer of the alc Gene
Expression System in Model and Crop Plant Species
Justin P.
Sweetman,1
Chengcai
Chu,
Nan
Qu,
Andrew J.
Greenland,*
Uwe
Sonnewald, and
Ian
Jepson
Crop Genetics Research, Syngenta, Jealott's Hill Research Station,
Bracknell, Berkshire RG42 6EY, United Kingdom (J.P.S., A.J.G., I.J.);
and Institut fur Pflanzengenetik und Kulturpflanzenforschung,
Correnstrasse 3, 06466 Gatersleben, Germany (C.C., N.Q., U.S.)
We have demonstrated that low concentrations of ethanol vapor
efficiently induce the alc gene expression system
in tobacco (Nicotiana tabacum cv Samsun NN), potato
(Solanum tuberosum cv Solara), and oilseed rape
(Brassica napus cv Westar). For many situations, this
may be the preferred method of induction because it avoids direct
application of comparatively high concentrations of an ethanol
solution. Although induction was seen with less than 0.4 µM ethanol vapor, maximal induction of the
chloramphenicol acetyl transferase gene was achieved after 48 h in
leaves of tobacco plants enclosed with 4.5 µM ethanol
vapor. In the absence of ethanol, there is no detectable gene
expression. Treatment of potato tubers with ethanol vapor results in
uniform  -glucoronidase (GUS) expression. Vapor treatment of a single
oilseed rape leaf resulted in induction of GUS in the treated leaf only
and 14C-ethanol labeling in tobacco confirmed that the
inducer was not translocated. In contrast, enclosure of the roots,
aerial parts, or whole plant with ethanol vapor resulted in induction
of GUS activity in leaves and roots. The data reported here broaden the utility of the alc system for research and crop biotechnology.
1
Present address: Genesis R&D, 1 Fox Street,
Parnell, Auckland, New Zealand.
*
Corresponding author; e-mail andy.greenland{at}syngenta.com; fax
44-1344-413638.
© 2002 American Society of Plant Physiologists
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