First published online July 18, 2002; 10.1104/pp.001149
Plant Physiol, August 2002, Vol. 129, pp. 1482-1493
The Expression of HAK-Type K+ Transporters Is
Regulated in Response to Salinity Stress in Common Ice
Plant1
Hua
Su,2
Dortje
Golldack,2
Chengsong
Zhao,3 and
Hans J.
Bohnert4*
Departments of Plant Sciences (H.S., H.J.B.), Biochemistry (C.Z.,
H.J.B.), and Molecular and Cellular Biology (H.J.B.), University of
Arizona, Tucson, Arizona 85721; and Lehrstuhl für
Stoffwechselphysiologie und Biochemie der Pflanzen, Universität
Bielefeld, Bielefeld, Germany (D.G.)
Four transcripts homologous to K+ transporters
of the HAK/KT/KUP family have been characterized from the common ice
plant (Mesembryanthemum crystallinum). We report
tissue-specific expression of McHAK1 and
McHAK4 transcripts abundant in roots, leaves, and stems.
McHAK2 was predominantly present in stems and
McHAK3 in root tissues. By in situ hybridizations, the
McHAKs showed signals in the leaf vascular bundles, mesophyll, and
epidermal cells as well as in epidermal bladder cells. In mature roots,
transcripts were mainly localized to the vasculature, and in
differentiated root tips, the strongest signals were obtained from the
epidermis. Expression of McHAK1, McHAK2,
and McHAK4 complemented a yeast mutant defective in low-
and high-affinity K+ uptake. Growth of the yeast mutant was
restored at low-millimolar K+ concentrations and was
inhibited by Rb+ and Cs+ but was not affected
by Na+. Transcript levels of McHAK1 and
McHAK4 increased by K+ starvation and by
salt stress of 400 mM NaCl in leaves and roots. Expression
of McHAK2 and McHAK3 was stimulated in
leaves and was transiently induced in roots in response to high
salinity with prestress transcript levels restored in salt-adapted
plants. We discuss possible roles for such transporters in ion
homeostasis at high salinity.
1
This work was supported by the Arizona
Agricultural Experiment Station and by the Deutsche
Forschungsgemeinschaft, Bonn (to D.G.).
2
These authors contributed equally to the work.
3
Present address: Anhui Academy of Agricultural Sciences,
Hefei, 230031, China.
4
Present address: Departments of Plant Biology and Crop
Sciences, University of Illinois, 1201 W. Gregory Drive, Urbana, IL 61801.
*
Corresponding author; e-mail bohnerth{at}life.uiuc.edu; fax
217-333-5574.
© 2002 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
C. Edelist, X. Raffoux, M. Falque, C. Dillmann, D. Sicard, L. H. Rieseberg, and S. Karrenberg
Differential expression of candidate salt-tolerance genes in the halophyte Helianthus paradoxus and its glycophyte progenitors H. annuus and H. petiolaris (Asteraceae)
Am. J. Botany,
October 1, 2009;
96(10):
1830 - 1838.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. S. Moller, M. Gilliham, D. Jha, G. M. Mayo, S. J. Roy, J. C. Coates, J. Haseloff, and M. Tester
Shoot Na+ Exclusion and Increased Salinity Tolerance Engineered by Cell Type-Specific Alteration of Na+ Transport in Arabidopsis
PLANT CELL,
July 1, 2009;
21(7):
2163 - 2178.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. R. Fulgenzi, M. L. Peralta, S. Mangano, C. H. Danna, A. J. Vallejo, P. Puigdomenech, and G. E. Santa-Maria
The Ionic Environment Controls the Contribution of the Barley HvHAK1 Transporter to Potassium Acquisition
Plant Physiology,
May 1, 2008;
147(1):
252 - 262.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-M. Wang, J.-L. Zhang, and T. J. Flowers
Low-Affinity Na+ Uptake in the Halophyte Suaeda maritima
Plant Physiology,
October 1, 2007;
145(2):
559 - 571.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Agarie, T. Shimoda, Y. Shimizu, K. Baumann, H. Sunagawa, A. Kondo, O. Ueno, T. Nakahara, A. Nose, and J. C. Cushman
Salt tolerance, salt accumulation, and ionic homeostasis in an epidermal bladder-cell-less mutant of the common ice plant Mesembryanthemum crystallinum
J. Exp. Bot.,
June 1, 2007;
58(8):
1957 - 1967.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Md. A. Kader, T. Seidel, D. Golldack, and S. Lindberg
Expressions of OsHKT1, OsHKT2, and OsVHA are differentially regulated under NaCl stress in salt-sensitive and salt-tolerant rice (Oryza sativa L.) cultivars
J. Exp. Bot.,
December 1, 2006;
57(15):
4257 - 4268.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Davies, R. Shin, W. Liu, M. R. Thomas, and D. P. Schachtman
Transporters expressed during grape berry (Vitis vinifera L.) development are associated with an increase in berry size and berry potassium accumulation
J. Exp. Bot.,
September 1, 2006;
57(12):
3209 - 3216.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Rodriguez-Navarro and F. Rubio
High-affinity potassium and sodium transport systems in plants
J. Exp. Bot.,
March 1, 2006;
57(5):
1149 - 1160.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Chinnusamy, A. Jagendorf, and J.-K. Zhu
Understanding and Improving Salt Tolerance in Plants
Crop Sci.,
January 31, 2005;
45(2):
437 - 448.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Ahn, R. Shin, and D. P. Schachtman
Expression of KT/KUP Genes in Arabidopsis and the Role of Root Hairs in K+ Uptake
Plant Physiology,
March 1, 2004;
134(3):
1135 - 1145.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. SHABALA
Regulation of Potassium Transport in Leaves: from Molecular to Tissue Level
Ann. Bot.,
November 1, 2003;
92(5):
627 - 634.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
