First published online July 18, 2002; 10.1104/pp.004762
Plant Physiol, August 2002, Vol. 129, pp. 1494-1506
Expression Analysis of a Family of nsLTP Genes Tissue
Specifically Expressed throughout the Plant and during Potato Tuber
Life Cycle1
Beatrix M.
Horvath,*
Christian W.B.
Bachem,
Luisa M.
Trindade,
Marian E.P.
Oortwijn, and
Richard G.F.
Visser
Graduate School Experimental Plant Sciences, Laboratory of
Plant Breeding, Department of Plant Sciences, Wageningen University,
6700 AJ Wageningen, P.O.B. 386 The Netherlands
Non-specific lipid-transfer proteins (nsLTPs) are
capable of binding lipid compounds in plant tissues and are coded by
the nsLTP genes. Here, we present the analysis of
expression of a family of potato (Solanum tuberosum)
nsLTP genes that express throughout the developing plant
in a highly tissue-specific manner. Three transcript-derived fragments
were isolated using an amplified restriction fragment
polymorphism-derived technique for RNA fingerprinting that show
homology to plant nsLTP genes. These transcript-derived fragments displayed modulated expression profiles related to the development of new tissues, with a peak of transcription around the
time of tuberization and just prior to sprout development, at dormancy
breakage. In addition, a homologous family of expressed sequence
tags was identified whose individual members could be classified
according to their tissue specificity. Two subgroups of expressed
sequence tags were found to express during tuber life cycle. To
study the regulation of potato nsLTP genes, two putative
potato nsLTP promoters were isolated and their
expression was studied using promoter-marker-gene fusions. The results
showed that one of the two promoters directed a highly specific pattern of expression detected in the phloem surrounding the nodes of young
plants and in the same tissue of tuber related organs, whereas the
second putative promoter showed little tissue or organ specificity. This difference in expression is likely due to a 331-bp insertion present in the tissue-specific promoter.
1
This research was supported in part by the
Technology Foundation Stichting Technische Wetenschappen (grant
no. WBI 4923).
*
Corresponding author; e-mail Beatrix.Horvath{at}wur.nl; fax
31-317-483457.
© 2002 American Society of Plant Physiologists
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