First published online August 1, 2002; 10.1104/pp.004754
Plant Physiol, August 2002, Vol. 129, pp. 1521-1532
A Novel Plant Homeodomain Protein Interacts in a Functionally
Relevant Manner with a Virus Movement Protein1
Bénédicte
Desvoyes,2
Sandrine
Faure-Rabasse,
Min-Huei
Chen,
Jong-Won
Park,3 and
Herman B.
Scholthof*
Department of Plant Pathology and Microbiology (B.D., S.F.-R.,
J.-W.P., H.B.S.) and Intercollegiate Faculty of Virology (H.B.S.),
Texas A&M University, 2132 TAMU, College Station, Texas 77843; and
Department of Biochemistry and Cell Biology, State University of New
York, Stony Brook, New York 11794-5215 (M.-H.C.)
Tomato bushy stunt virus and its cell-to-cell
movement protein (MP; P22) provide valuable tools to study trafficking
of macromolecules through plants. This study shows that wild-type P22
and selected movement-defective P22 amino acid substitution mutants
were equivalent for biochemical features commonly associated with MPs
(i.e. RNA binding, phosphorylation, and membrane partitioning). This
generated the hypothesis that their movement defect was caused by
improper interaction between the P22 mutants and one or more host
factors. To test this, P22 was used as bait in a yeast
(Saccharomyces cerevisiae) two-hybrid screen with
a tobacco (Nicotiana tabacum) cDNA library, which
identified a new plant homeodomain leucine-zipper protein that
reproducibly interacted with P22 but not with various control proteins.
These results were confirmed with an independent in vitro binding
test. An mRNA for the host protein was detected in plants, and its
accumulation was enhanced upon Tomato bushy stunt virus
infection of two plant species. The significance of this interaction
was further demonstrated by the failure of the homeodomain
protein to interact efficiently with two of the well-defined movement-deficient P22 mutants in yeast and in vitro. This is the first
report, to our knowledge, that a new plant homeodomain leucine-zipper
protein interacts specifically and in a functionally relevant manner
with a plant virus MP.
1
This work was supported by the Texas
Agricultural Experiment Station (grant no. TEX08387), by the U.S.
Department of Agriculture/CSREES-National Research Initiative
Competitive Grants Program (grant no. 99-35303-8022), by the Texas
Higher Education Coordinating Board Advanced Technology Program (grant
no. 000517-0070-1999), and by the S.R. Noble Foundation, Inc.
2
Present address: Centro de Biologia Molecular "Severo
Ochoa" Universidad Autonoma de Madrid Cantoblanco, 28049 Madrid, Spain.
3
Present address: Virus Research, John Innes Centre,
Colney Lane, Norwich NR4 7UH, UK.
*
Corresponding author; e-mail herscho{at}tamu.edu; fax 979-845-6483.
© 2002 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
D. Hofius, A. T. Maier, C. Dietrich, I. Jungkunz, F. Bornke, E. Maiss, and U. Sonnewald
Capsid Protein-Mediated Recruitment of Host DnaJ-Like Proteins Is Required for Potato Virus Y Infection in Tobacco Plants
J. Virol.,
November 1, 2007;
81(21):
11870 - 11880.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. T. Omarov, J. J. Ciomperlik, and H. B. Scholthof
RNAi-associated ssRNA-specific ribonucleases in Tombusvirus P19 mutant-infected plants and evidence for a discrete siRNA-containing effector complex
PNAS,
January 30, 2007;
104(5):
1714 - 1719.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. P. Brizard, C. Carapito, F. Delalande, A. Van Dorsselaer, and C. Brugidou
Proteome Analysis of Plant-Virus Interactome: Comprehensive Data for Virus Multiplication Inside Their Hosts
Mol. Cell. Proteomics,
December 1, 2006;
5(12):
2279 - 2297.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Xiang, K. Kakani, R. Reade, E. Hui, and D. Rochon
A 38-amino-Acid sequence encompassing the arm domain of the cucumber necrosis virus coat protein functions as a chloroplast transit Peptide in infected plants.
J. Virol.,
August 1, 2006;
80(16):
7952 - 7964.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Luo, F. Song, and Z. Zheng
Overexpression in transgenic tobacco reveals different roles for the rice homeodomain gene OsBIHD1 in biotic and abiotic stress responses
J. Exp. Bot.,
October 1, 2005;
56(420):
2673 - 2682.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. S. Nelson and V. Citovsky
Plant Viruses. Invaders of Cells and Pirates of Cellular Pathways
Plant Physiology,
August 1, 2005;
138(4):
1809 - 1814.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. F. Carvalho and S. G. Lazarowitz
Interaction of the Movement Protein NSP and the Arabidopsis Acetyltransferase AtNSI Is Necessary for Cabbage Leaf Curl Geminivirus Infection and Pathogenicity
J. Virol.,
October 15, 2004;
78(20):
11161 - 11171.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Gomez and V. Pallas
A Long-Distance Translocatable Phloem Protein from Cucumber Forms a Ribonucleoprotein Complex In Vivo with Hop Stunt Viroid RNA
J. Virol.,
September 15, 2004;
78(18):
10104 - 10110.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Kragler, M. Curin, K. Trutnyeva, A. Gansch, and E. Waigmann
MPB2C, a Microtubule-Associated Plant Protein Binds to and Interferes with Cell-to-Cell Transport of Tobacco Mosaic Virus Movement Protein
Plant Physiology,
August 1, 2003;
132(4):
1870 - 1883.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-W. Park, B. Desvoyes, and H. B. Scholthof
Tomato Bushy Stunt Virus Genomic RNA Accumulation Is Regulated by Interdependent cis-Acting Elements within the Movement Protein Open Reading Frames
J. Virol.,
November 13, 2002;
76(24):
12747 - 12757.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
